This study was conducted to evaluate the effect of frozen storage time (30, 60, 90 or 180 days) and cooking (100 °C, 30 min) on the physical characteristics and oxidative stability of M. Gastrocnemius pars interna (GN) and M. Iliofiburalis (IF) of rhea americana. Physical parameters measured included thawing and cooking loss, colour parameters (L*a*b*), while oxidation was assessed by determini...

NSCs/NPCs could be used for Sensorineural hearing loss treatment, because of the extensive capacity for self-renewal and pluripotency. In order to isolate and identify neural precursor cells (NPCs), we established a strategy to isolate and cultivate NPCs. Immunohistochemistry, immunofluorescence, Western blotting, and electron microscopy were used to characterize the cells and compare their dif...

this paper is based on an experimental research that examined the effects of concrete`s major parameters on durability of concrete pavements and curbs under freezing and thawing cycles. these parameters include concrete mix design parameters such as water-cement ratio, fine aggregate percentage and using air entraining admixture and simulating real freeze-thaw cycles that infrastructures underg...

The addition of graphene oxide (GO) and fly ash (FA) to SFRC (steel fiber reinforced concrete) increases frost resistance. Based on the analysis amelioration GO resistance SFRC, improvement mechanism effects FA durability were studied in depth. test blocks’ compressive strength, relative dynamic modulus elasticity, mass-loss rate tested through experiments. pore distribution morphological chara...

In a recent review on the storage of animal viruses at subzero temperatures (8), it was pointed out that enveloped viruses present special problems. For example, herpesvirus even at -90 C loses infectivity after prolonged storage and at -20 C is even more labile than at 4 C. Repeated freezings and thawings of this virus result in marked loss in activity. Members of the herpesvirus group can be ...

Sperm cryopreservation, in combination with assisted reproductive techniques, is a valuable tool for the genetic management of endangered felids. However, the acrosome of the cheetah spermatozoon is especially sensitive to cryopreservation, with approximately 40% of spermatozoa experiencing acrosomal damage immediately after thawing and then another approximately 15% loss during the next 4 hour...

Cryopreservation of seabass (Lates calcarifer) semen is an approach to increase the quantity and quality of seabass fry in aquaculture. However, cold shock can induce sperm injury leading to structural damage of the plasma membrane and loss of motility. Thus, the effect of cryopreservation on fatty acid composition and antioxidant enzyme activities of seabass sperm was determined. In cryopreser...