A hyperthermophilic archaeon Pyrococcus horikoshii was isolated from a hydrothermal vent in the Okinawa trough in the Pacific Ocean. It optimally grows at around 100oC. The hyperthermophiles were expected to be good producers of thermostable enzymes active at around 100 oC. The thermostable enzymes have considIkuo MATSUI Biological Information Research Center e-mail: [email protected] AIST T...

We describe a method to genetically manipulate Chaetomium thermophilum, a eukaryotic thermophile, along with various biochemical applications. The transformation method depends on a thermostable endogenous selection marker operating at high temperatures combined with chromosomal integration of target genes. Our technique allows exploiting eukaryotic thermophiles as source for purifying thermost...

About thirty-five percent of coffee pulp waste is pectin. It may potentially be a source to used in the bioprocessing industry. For example, it can as substrate produce pectinase from microorganisms under solid-state fermentation (SSF). In this investigation, an isolated fungus VTM4 with density 10 7 spores/mL was grown on medium-based, and after 0-168 hours incubation at 30 °C, activity detect...

A degumming approach is used in this paper with alkalophilic pectinase-producing bacteria (APPB) and two sources of water solvents to address the existing conventional retting complexities kenaf. The incorporation APPB was confirmed based on their feasibilities multiple cell-wall-degrading enzymatic delicacy. combinations seawater offered achievements within six-day non-sterile conditions. Thes...

Pectinases are widely used in food processing, textile and other allied industries. Cassava peels generated from cassava processing outfits constitutes one of the major environmental pollutions Nigeria there is need to find alternative economic value waste instead indiscriminately disposing them as currently done. This study reports optimization pectinase production Bacillus subtilis PSE-8 usin...

Aspergillus spp. are widely spread plant pathogens. In several kinds of fruit, especially in apple and peach, aspergillus rot is one of the postharvest desease in fruit, but the infection mechanism is not known. The degradation of cell walls by pathogens is significant in primary infection and afterinfection, and plays an important role in the advancement of mycelium into the host plant and the...

Changes in the soluble protein content, electrophoretic profiles of total and thermostable proteins and polypeptides in endosperms and roots of wheat seedlings germinated under optimal and stress conditions were investigated. Low and high temperature, 0.2 M NaCl, 0.5 M sucrose, 30 μM ABA and 10 mM H2O2 were applied as stress factors and seeds germinated for 72 hours. The results obtained show t...

The ability to design thermostable proteins offers enormous potential for the development of novel protein bioreagents. In this work, a combined computational and experimental method was developed to increase the T m of the flavin mononucleotide based fluorescent protein Bacillus Subtilis YtvA LOV domain by 31 Celsius, thus extending its applicability in thermophilic systems. Briefly, the metho...

As natural fibers, low-cost biosorbents have proven to be an effective and clean tool remove textile dyes from wastewater. In this research, the Reactive Black 5 removal ability of original enzyme-modified fibers were assessed. A fiber extracted a Colombian fique plant (Furcraea sp.) was employed. The effects protonation with different solvents dye solution pH on RB5 evaluated. biosorbent chemi...

BACKGROUND Thermostable enzymes have several benefits in lignocellulose processing. In particular, they potentially allow the use of increased substrate concentrations (because the substrate viscosity decreases as the temperature increases), resulting in improved product yields and reduced capital and processing costs. A short pre-hydrolysis step at an elevated temperature using thermostable en...