Sir, The letter by Haack et al. (2014) provides important information confirming the role of the thiamine transporter hTHTR2 in Leigh syndrome and the beneficial effect of biotin and/or thiamine treatment for patients harbouring mutations in the gene encoding hTHTR2, SLC19A3. In recent years many new pathogenic mutations have been reported in SLC19A3 resulting in several age-related neurologica...

In recent years, a series of thiamine derivatives has been synthesized and many investigations have been made both chemically and medically. Allithiamine, a S-alkyl thiamine derivative, was studied in the previous pa per (1) and was found to have polarographically a similar behavior to thia mine. In the present experiment, polarographic investigations on diacetyl thiamine (DAT) and dibenzoylthi...

The present study examined the intestinal uptake of thiamine (vitamin B1) using the human-derived intestinal epithelial cells Caco-2 as an in vitro model system. Thiamine uptake was found to be 1) temperature and energy dependent and occurred with minimal metabolic alteration; 2) pH sensitive; 3) Na+ independent; 4) saturable as a function of concentration with an apparent Michaelis-Menten cons...

The worldwide prevalence of diabetes has risen dramatically over the past two decades from an estimated 30 million cases in 1985 to 382 million in 2013. The traditional anti diabetic drugs have several drawbacks in long term use. Thiamine is a member of the vitamin B family. Thiamine is necessary for normal insulin synthesis and secretion. So it may be beneficial in diabetic persons where insul...

Thiamine deficiency is a condition that affects millions of infants in South and Southeast Asia due to heavy cultural reliance on thiamine-poor, polished white rice as dietary staple. Previous research suggests thiamine-deficient diet not only endangers infants’ health, but also hinders neuro-cognitive development. As part larger, randomized controlled trial, this thesis investigated possible b...

BACKGROUND Thiamine is a vitamin that is essential for adequate aerobic metabolism. The objective of this study was to determine if thiamine administration prior to coronary artery bypass grafting would decrease post-operative lactate levels as a measure of increased aerobic metabolism. METHODS We performed a randomized, double-blind, placebo-controlled trial of patients undergoing coronary a...

Thiamine is a key primary metabolite which is necessary for the viability of all organisms. It is a dietary requirement for mammals because only prokaryotes, fungi and plants are thiamine prototrophs. In contrast to the well documented biosynthetic mechanism in bacteria, much remains to be deciphered in plants. In this work, a tomato thiamine-auxotrophic (thiamineless, tl) mutant was characteri...

The responses of plants to abiotic stress involve the up-regulation of numerous metabolic pathways, including several major routes that engage thiamine diphosphate (TDP)-dependent enzymes. This suggests that the metabolism of thiamine (vitamin B1) and its phosphate esters in plants may be modulated under various stress conditions. In the present study, Zea mays seedlings were used as a model sy...

Abstract In vitro propagation of Plant Genetic Resources is a basic step for routine genebank and biotechnology research activities. Accelerating growth rooting in plants contributes to an improvement process efficiency plant quality. the present study effect supplemental thiamine explant size on biometric variables, ion content sap, chlorophyll leaves moisture were assessed replicated trial gr...

In a multiple change-over study the bioequivalence of 3 thiamine preparations, used therapeutically as neurotropic agents for the treatment of polyneuropathies, was tested in a collective of 7 volunteers. After ingestion of a single dose of either 100 mg benfotiamin CS-benzoylthiamine-o-monophosphate), fursultiamin (thiamintetrahydrofurfuryldisulfide) or thiaminedisulfide, thiamine blood levels...