In an earlier study (S. W. Jordan and J. E. Cronan, Jr., J. Biol. Chem. 272:17903-17906, 1997) we reported a new enzyme, lipoyl-[acyl carrier protein]-protein N-lipoyltransferase, in Escherichia coli and mitochondria that transfers lipoic acid from lipoyl-acyl carrier protein to the lipoyl domains of pyruvate dehydrogenase. It was also shown that E. coli lipB mutants lack this enzyme activity, ...

The lux-specific acyltransferases are serine esterases responsible for preferential diversion of myristic acid from fatty acid biosynthesis to the luminescent system. In contrast to other acyltransferases, an acylated enzyme intermediate can readily be detected making it ideal for the study of the mechanism of acyl transfer. Although the transferase readily cleaves acyl carrier protein and acyl...

The specificity and reactivity of complement serine proteases D, B, Bb, C2, and C2a were determined using a series of peptide thioester substrates. The rates of thioester hydrolysis were measured using assay mixtures containing the thiol reagent I,lt’-dithiodipyridine at pH 7.5. Each substrate contained a PI arginine residue, and the effect of various groups and amino acids in the Pz, P3, P4, a...

Homogeneous preparations of fatty acid synthetase from mammary glands of lactating rats were used to prepare an antiserum and a y-globulin fraction was isolated from the antiserum. The antiserum and the y-globulin fraction were shown to be specific against the fatty acid synthetase multienzyme complex. Addition of the y-globulin to homogenate fractions of lactating rat mammary glsnds, resulted ...

Salts activate the fatty acid-synthesizing system of Escherichia coli and several of its component enzymes. The 3hydroxyacyl coenzyme A dehydrogenase from pig heart, one of the enzymes of fatty acid oxidation, is also stimulated by salts. Although all cations examined produce this stimulation, the activation patterns caused by monovalent cations are clearly distinct from those of divalent catio...

Organisms such as bacteria, fungi, viruses, and nematodes cause yield quality loss in agricultural production. Especially the control of fungal pathogens is challenging. The fact that chemical pesticides used these are not environmentally friendly, increases importance alternative methods use biopesticides. Biopesticides relatively harmful to human health environment generally secondary metabol...