Cyanobacteria have been engineered to produce ethanol through recent synthetic biology efforts. However, one major challenge to the cyanobacterial systems for high-efficiency ethanol production is their low tolerance to the ethanol toxicity. With a major goal to identify novel transporters involved in ethanol tolerance, we constructed gene knockout mutants for 58 transporter-encoding genes of S...

INCORPORATING GEOMETRIC FEATURE VARIATION WITH KINEMATIC TOLERANCE ANALYSIS OF 3D ASSEMBLIES Jeffrey G. Dabling Department of Mechanical Engineering Master of Science As technology increases and performance requirements continually tighten, the cost and required precision of assemblies increase as well. There exists a strong need for increased attention to tolerance analysis to enable high-prec...

Statistical tolerance analysis based on Monte Carlo simulation can be applied to obtain a cost-optimized specification that satisfies both the cost and quality requirements associated with manufacturing. However, this process requires time-consuming computations. We found an implementation uses graphics processing unit (GPU) for vector-chain-based statistical scales better increasing sample siz...