Temporal restriction of feeding can entrain circadian behavioral and physiological rhythms in mammals. Considering the critical functions of the hypothalamic orexin (hypocretin) neuropeptides in promoting wakefulness and locomotor activity, we examined the role of orexin neurons in the adaptation to restricted feeding. In orexin neuron-ablated transgenic mice, the food-entrained rhythmicity of ...

conclusions the canola oilseeds could provide a useful expression system to produce hcv core protein as a vaccine candidate. results western blot analysis using anti-his antibody confirmed the presence of a 15 kda protein in the seeds of t1 transgenic lines. the amount of antigenic protein accumulated in the seeds of these transgenic lines was up to 0.05% of the total soluble protein (tsp). mat...

background: leishmaniasis is a worldwide disease prevalent in tropical and sub- tropical countries. in the present study the immunogenicity of three human hla-dr1 restricted peptides derived from l. major gp63 protein was evaluated using fvb/n-dr1 transgenic mouse model. methods: the immunity generated by three mhc class ii – restricted peptides with the sequence of aarlvrlaaagaavt (aar), aaplv...

The Crop-Livestock Integration system has sustainable potential. But pests such as the defoliating caterpillar Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae) can reduce productivity of this system. objective was to report transgenic maize and Brachiaria brizantha (A. Rich.) (Poaceae) plants attacked by S. in Feroz VIP3 ® (SYN8A98 TLTG Viptera) B. MG-5 Xaraés were evaluated 20 days...

Froy, Oren, Nava Chapnik, and Ruth Miskin. Long-lived MUPA transgenic mice exhibit pronounced circadian rhythms. Am J Physiol Endocrinol Metab 291: E1017–E1024, 2006. First published June 20, 2006; doi:10.1152/ajpendo.00140.2006.—Robust biological rhythms have been shown to affect life span. Biological clocks can be entrained by two feeding regimens, restricted feeding (RF) and caloric restrict...

Impedimetric analysis method is an important tool for food safety detection. In this work, a novel impedimetric microfluidic analysis system consisted of a printed gold electrode chip and a microfluidic flow cell was developed for sensitive and selective detection of transgenic protein Cry1Ab. Anti-Cry1Ab aptamer coated magnetic beads were used to recognize transgenic protein Cry1Ab and form Cr...

Oral administration of soluble protein Ags typically induces Ag-specific systemic nonresponsiveness. However, we have found that feeding a model food protein, OVA, to helminth-infected mice primes for a systemic OVA-specific Th2 response. In this report we show that, in addition to creating a Th2-priming cytokine environment, helminth infection up-regulates costimulatory molecule expression on ...

Agouti-related protein (AgRP) and neuropeptide Y (NPY) are colocalized in arcuate nucleus (arcuate) neurons implicated in the regulation of energy balance. Both AgRP and NPY stimulate food intake when administered into the third ventricle and are up-regulated in states of negative energy balance. However, mice with targeted deletion of either NPY or AgRP or both do not have major alterations in...

Large parts of agricultural soil are contaminated with lead (Pb) and cadmium (Cd). Although most environments are not heavily contaminated, the low levels observed nonetheless pose a high risk of heavy metal accumulation in the food chain. Therefore, approaches to develop plants with reduced heavy metal uptake are important. Recently, many transgenic plants with increased heavy metal resistance...

Rice (Oryza sativa L.) is a primary global food cereal. However, when compared to wheat, rice has poor food processing qualities. Dough that is made from rice flour has low viscoelasticity because rice seed lacks storage proteins that are comparable to gluten protein from wheat. Thus, current research efforts aim to improve rice flour processing qualities through the transgenic expression of vi...