rna extraction is a prerequisite technique for gene expression studies, analyzing the etiology and disease progression, treatment effects, as well as designing the diagnostic methods. although many rna extraction kits have been commercialized, but these kits are expensive and are not accessible in some countries. many studies have shown that trizol is an applicable material for the rna extracti...

abstract: a pure nucleotide pool is an essential precursor of correct dna replication and prevention of mutagenesis and abnormality in a living cell. inosine triphosphatase (itpase) is a critical enzyme to remove any deaminated rough purine nucleotides such as inosine from nucleotide pool. it has been shown that the rate of substitution mutation in genomic dna increase by abnormal function and ...

Kernel culture was assessed for evaluating novel gene expression in developing maize (Zea mays L.) seeds by comparing the transient expression of maize ubiquitin (Ubi-I) promoter-driven ,B-glucuronidase (GUS) delivered by particle bombardment in kernels grown in culture with those grown in planta. With kernels from either source, GUS expression, as determined by histochemical staining, was wide...