Clones including tRNA genes from tomato {Lycopersicon esculentum) chloroplast DNA have been obtained. Here we report the nucleotide sequence of tmH\ trnD-^ and tmL\ Sequences were determined by the dideoxy method (1). In higher plant chloroplast genomes, tmY and trnD are clustered together with trnE in the large single copy (LSQ region (2). The sequence of trnY reveals 100% identity with its co...

Cucurbitaceae contain c. 800 species in 130 genera and are among the economically most important families of plants. We inferred their phylogeny based on chloroplast DNA sequences from two genes, one intron, and two spacers (rbcL, matK, trnL, trnL-trnF, rpl20-rps12) obtained for 171 species in 123 genera. Molecular data weakly support the traditional subfamilies Cucurbitoideae (111 genera) and ...

BACKGROUND Species identification of living organisms by standard DNA sequences has been well-accepted. Consortium for the Barcode of Life (CBOL) recommends chloroplast regions rbcL and matK as the DNA barcodes for the land plants. This study aims to evaluate the feasibility and limitations of rbcL, matK, and 5 other commonly used regions as the DNA barcodes for the medicinal Gentiana and their...

A phylogenetic study of the Opopanax and Crenosciadium was conducted to elucidate their circumscription infrageneric relationships. Sequence data from nrDNA ITS, cpDNA matK trnL-F were used determine relationship. Polymerase Chain Reaction (PCR) amplifications all regions performed using universal primers. Maximum Likelihood (ML) trees constructed PAUP* software, another tree ITS sequences show...

The identity of Ceropegia hirsuta in Thailand has been questioned. Although this plant is quite similar to the original C. from India, being entirely hirsute, with yellowish-shaded flowers, a curved, funnel-shaped corolla tube, and broad lobes shorter than there are several clear differences morphological traits between materials collected type further specimens India. Moreover, s.s. restricted...

Thlaspi arvense is a well-known annual farmland weed with worldwide distribution, which can be found from sea level to above 4000 m high on the Qinghai-Tibetan Plateau (QTP). In this paper, a phylogeographic history of T. arvense including 19 populations from China was inferred by using three chloroplast (cp) DNA segments (trnL-trnF, rpl32-trnL and rps16) and one nuclear (n) DNA segment (Fe-reg...

Eight new rbcL DNA sequences and 15 new sequences from the 5' end of the chloroplast ndhF gene were obtained from representative Dipsacales and outgroup taxa. These were analyzed in combination with previously published sequences for both regions. In addition, sequence data from the entire ndhF gene, the trnL-F intergenic spacer region,the trnL intron,the matK region, and the rbcL-atpB intergen...

Intra- and interspecific phylogenetic relationships of the rare serpentine endemic taxon Caulanthus amplexicaulus var. barbarae and related taxa in the "Streptanthoid Complex" of genera (Streptanthus, Caulanthus, Guillenia) were examined using nuclear ribosomal internal transcribed spacer (ITS) and chloroplast trnL intron sequences. Phylogenetic hypotheses generated from 81 variable ITS nucleot...

Phylogenetic relationships within Limonium (Plumbaginaceae) are evaluated using sequence data from three plastid regions (rbcL, the trnL intron, and the trnL-trnF intergenic spacer). Sixty-six species representing the major genera of Staticoideae, including representatives of all sections and genera formerly included in Limonium, have been analyzed using four species of Plumbaginoideae as an ou...

Stemona sessilifolia, S. japonica and S. tuberosa are the three genuine sources of Stemonae Radix specified in the Chinese Pharmacopoeia (CP) for antitussive and insecticidal remedy. Significant variations in alkaloids composition and content, as well as different degree of antitussive activity were found among them. In order to accurately identify the genuine sources of Stemonae Radix in the g...