Many proteins have been shown to cap the fast growing (barbed) ends of actin filaments, but none have been shown to block elongation and depolymerization at the slow growing (pointed) filament ends. Tropomodulin is a tropomyosin-binding protein originally isolated from red blood cells that has been localized by immunofluorescence staining to a site at or near the pointed ends of skeletal muscle...

Myofibrils in vertebrate cardiac and skeletal muscles are characterized by groups of proteins arranged in contractile units or sarcomeres, which consist of four major components - thin filaments, thick filaments, titin and Z-bands. The thin actin/tropomyosin-containing filaments are embedded in the Z-bands and interdigitate with the myosin-containing thick filaments aligned in A-bands. Titin is...

Household arthropods are one of the most common causes of allergic diseases. Four species of cockroaches are found to reside in Korean homes, but published work deals almost exclusively with the German and American cockroaches. This study was undertaken to investigate the cross-reactive allergenic components of the dusky brown cockroach, Periplaneta fuliginosa. Enzyme-linked immunosorbent assay...

During muscle contraction, a molecular interaction takes place between the myofilament proteins actin and myosin, which is triggered by a rise in intracellular calcium and is driven by the energy from ATP hydrolysis. The tropomyosin– troponin complex inhibits the actin–myosin interaction at low intracellular-free calcium. This inhibition is released when intracellular-free calcium increases and...

boophilus genus ticks are responsible for transferring some pathogens and reducing production factors in cattle. tropomysin (tpm) protein has actin regulator activity and playing important role in immune and allergic reactions. the main goal is to determine different aspects of phylogenetic, similarity, homology, structure and allergenicity of tpm protein. in prior study, we identified tpm by u...

Background Food allergy is apparently increasing and it is estimated that 3–4% of adults have food allergy. Shrimp is one of the most common causatives in seafood allergy. Patients with shrimp allergy may exhibit life threatening anaphylactic reactions. Tropomyosin is a known important allergen in shrimp. The IgE-binding epitope in shrimp tropomyosin, cross-reacts with other allergenic inverteb...

An antibody against purified chicken skeletal muscle tropomyosin is used in indirect immunofluorescence to visualize the localization of tropomyosin in a variety of nonmuscle cells. The antibody produces a fluorescent pattern which is very similar to that obtained with an actin-specific antibody. This pattern is composed of fluorescent fibers which are shown to be coincident with the fibers see...

The fraction of polyclonal caldesmon antibodies cross-reacting with rabbit skeletal troponin T are shown to compete with smooth muscle tropomyosin for caldesmon and troponin T, as revealed by ELISA method. The epitope recognized by these antibodies was also found in Mr 77 kDa non-muscle caldesmon. These results provide functional confirmation for the suggestion that the regions of amino acid se...