OBJECTIVE Trypan blue and fluorescein sodium are two commonly used dyes in ophthalmic practice. The staining of anterior lens capsule is achieved using trypan blue while fluorescein finds its application in fluorescent angiography. We assessed the 'in vitro' bactericidal effect of these two agents. MATERIALS AND METHODS The cidal properties of these two dyes were tested in vitro against thirt...

Suspensions of islet cells were prepared by shaking pancreatic islets from non-inbred ob/ob mice in a Ca2+-free buffer. The cells were incubated with or without 20 mM-alloxan, and subsequently with Trypan Blue. The uptake of Trypan Blue by cell nuclei was analysed by microscope photometry and by counting the frequency of cells appearing stained on visual inspection. Cells classified as stained ...

THEt eratogenic activity of several disazo dyes during rat embryogenesis has been investigated by Wilson (1955). In addition to the marked teratogenicity of trypan blue Wilson also demonstrated that Evans blue was moderately active and that Niagara blue 4B and Niagara sky blue 6B were possibly active. On the other hand, several other closely related disazo dyes such as Congo red and azo blue we...

Pregnant rats were injected intraperitoneally with a teratogenic dose of trypan blue (14 mg/100 g or 20 mg/100 g body weight) on the eighth day of gestation. Fetal blood was collected from control and experimental fetuses on the twentieth day of pregnancy. Serum was obtained from pooled blood samples of two to three fetuses. Paper electrophoresis was carried out in a Spinco model R system using...

Osteosarcoma is the most commonly diagnosed malignant bone tumor in humans, with a higher incidence children and young people. It highly aggressive has high metastatic potential. Its treatment based on both chemotherapy surgical intervention. However, currently used chemotherapeutic agents, such as doxorubicin, have several adverse effects patient. Therefore, there growing demand for new agents...