BACKGROUND Widely used restriction-dependent cloning methods are labour-intensive and time-consuming, while several types of ligase-independent cloning approaches have inherent limitations. A rapid and reliable method of cloning native DNA sequences into desired plasmids are highly desired. RESULTS This paper introduces ABI-REC, a novel strategy combining asymmetric bridge PCR with intramolec...

The presented paper, which is the first of two parts, shows results numerical investigations a heat exchanger channel in form cylindrical tube with thin insert. insert, placed concentrically pipe, uses phenomenon thermal radiation absorption to intensify transfer between pipe wall and gas. Eight geometric configurations insert size were numerically investigated using CFD software, varying its d...

The human chemokine receptor (CKR) genes CCR2, CCR6, CCR7, CCR9, CCR10, CXCR4, and CXCR5 harbor one or two introns. CCR7, CCR9, CCR10, and CXCR5 introns, (but not CCR2, CCR6, and CXCR4 introns) encompass retrovirus-like inserts with the characteristics of SINEs (short interspersed nuclear elements) up to 300 nucleotides (nt) long. Other characteristic elements of the retroviral genome, such as ...

The present experimental work is performed to investigate the convection heat transfer (HT), pressure drop (PD), irreversibility, exergy efficiency and thermal performance for turbulent flow inside a uniformly heated circular channel fitted with novel geometry of hybrid tape. Air taken as working fluid Reynolds number varied from 10,000 80,000. Hybrid tape made up combination grooved spring wav...

The article deals with the rationalization of production shaped inserts for casting rotors. theoretical part describes characteristics rotor casting, die technology, analysis existing technology inserts. main is focused on proposed technological process production, comparison and process. concludes a technical economic benefit solution evaluation work. Rationalization has its justification in d...

The results of an experimental study the combined enamelling technique are presented – enamel with granulation. Original inserts, decorated granules various colours and sizes, were obtained, use which expands possibilities decorative enamelling. It is shown that different degrees fusion grain on prepared base gives artistic effects. Spherical grains, qualitatively adhered to ground, give a more...

A microchannel heat exchanger effectively evacuates from a confined space. This paper attempts to gain insight into the combinatorial repercussions of simultaneously coupling two factors that affect microchannel’s performance, which channel size and micro-insert complexity are main contributors. With water as working fluid, an ANSYS-based numerical analysis was carried out for distinct sizes, 1...

   Prolonged residence of drug formulation in the nasal cavity is important for the enhancing intranasal drug delivery. The objective of the present study was to develop a mucoadhesive in-situ gelling nasal insert which would enable the reduced nasal mucociliary clearance in order to improve the bioavailability of metoclopramide hydrochloride. Metoclopramide hydrochloride is a potent antiemetic...

Aim: Patients can be protected against possible complications when antihypertensive drugs are regularly and properly used for the treatment of hypertension. The readability package inserts increases compliance. In present study, purpose was to determine level inserts.
 Materials Method: A total 64 commonly were selected this study. scores calculated according Readability Scales developed b...