We described a method for PCR amplification of unknown flanking genomic DNA fragments. This method is a combination of PCR with "endtr imming method" and "cassettes and cassette-primers method". In this method, genomic DNA was digested with three different groups of restriction enzymes. DNA in group 1 was digested with BamHI, Bglll, Fbal, or Mbol. DNA in group 2 was digested with Blnl, Nhel, Sp...

BACKGROUND Specific and sensitive nucleic acid (NA) testing in research and clinical diagnostics is usually performed by use of labeled oligonucleotide probes. However, the use of target-specific fluorogenic probes increases the cost of analysis. Therefore, universal sequence-dependent (USD) NA detection methods have been developed to facilitate cost-effective target detection using standardize...

Based on comparative phylogenetic analysis of 16S rRNA gene sequences deposited in an RDP database, we constructed a local database of thaumarchaeotal 16S rRNA gene sequences and developed a novel PCR primer specific for the archaeal phylum Thaumarchaeota. Among 9,727 quality-filtered (chimeral-checked, size >1.2 kb) archaeal sequences downloaded from the RDP database, 1,549 thaumarchaeotal seq...

Increasing evidence of the fungal diversity in deep-sea sediments has come from amplification of environmental DNA with fungal specific or eukaryote primer sets. In order to assess the fungal diversity in deep-sea sediments of the Central Indian Basin (CIB) at ~5,000 m depth, we amplified sediment DNA with four different primer sets. These were fungal-specific primer pair ITS1F/ITS4 (internal t...

objective: the objective of this study was to compare the tensile bond strength of metal brackets bonding to glazed ceramic surfaces using three various surface treatments. materials and methods: forty two glazed ceramic disks were assigned to three groups. in the first and second groups the specimens were etched with 9.5% hydrofluoric acid (hfa). subsequently in first group, ceramic primer and...

in 2011-2012 surveys for phytoplasma diseases, eggplant )solanum melongena l.( plants with phyllody symptoms were observed in eggplant fields of roodan (hormozgan province of iran). agent of roodan eggplant phyllody (rep) was transmitted from phyllody affected eggplant to eggplant and tomato by grafting and to periwinkle via dodder inoculation inducing phytoplasma-type symptoms. phytoplasmal in...

Aichi viruses (AiVs) have been proposed as a causative agent of human gastroenteritis potentially transmitted by fecal-oral routes through contaminated food or water. In the present study, we developed a TaqMan minor groove binder (MGB)-based reverse transcription-quantitative PCR (RT-qPCR) system that is able to quantify AiVs and differentiate between genotypes A and B. This system consists of...