The urinary metabolites of N-2-fluorenylacetamide were separated by chromatography on an alumina column into three classes: (a) unconjugated materials, amounting to 5-10 per cent of the urinary activity; (b) sulfuric acid conjugates (20-25 per cent); (c) glucuronic acid conjugates (60-70 per cent). Evidence for the structure of these materials was adduced by solvent partition, paper chromatogra...

BACKGROUND Few data exist in Latin America concerning the association between organophosphate (OP) urinary metabolites and the consumption of fruits and vegetables and other exposure risk variables in schoolchildren. METHODS We collected samples of urine from 190 Chilean children aged 6-12 years, fruits and vegetables, water and soil from schools and homes, and sociodemographic data through a...

Calcium oxalate crystals are responsible for formation of urinary calculi. Traditional therapy based on plants and herbal resources have found beneficial treatment kidney stones. The present study aimed to screen antilithiatic antioxidant potential Polyalthia Longifolia leaves methanolic extract. extract was evaluated the presence secondary metabolites, total phenolic content flavonoid contents...

Charcoal is an important source of energy for domestic and industrial use in many countries. Brazil is the largest producer of charcoal in the world, with approximately 350,000 workers linked to the production and transportation of charcoal. To evaluate the occupational exposure to wood smoke and potential genotoxic effects on workers in charcoal production, we studied urinary mutagenicity in S...

Two polar metabolites of mitoxantrone, a clinically active antitumor agent, have been isolated and purified from the urine of patients by sequential absorption on glass wool and Cu-Sep-Pak cartridges followed by preparative high-performance liquid chromatography. Negative ion chemical ionization mass spectrometry indicated that the two metabolites are the diand mono-carboxylic acids resulting f...

Urinary excretion of tryptophan and niacin metabolites was measured before and after oral loads of 2.0 g L-tryptophan in 12 normal smokers, in the same subjects after having stopped smoking for 3 weeks, and again after resumption of smoking. The metabolites measured were kynurenine, hydroxykynurenine, kynurenic acid, xanthurenic acid, acetylkynurenine, o-aminohippuric acid, anthranilic acid glu...

OBJECTIVE The alteration of steroid hormonal status in premenopausal breast disease (benign and malignant) were investigated by comparing the urinary profile of androgens and corticoids. METHODS The urinary concentrations of 25 androgens and corticoids were quantitatively determined by a gas chromatographymass spectrometry system in patients with benign breast disease (35 cases, 20-54 years),...

Triethylenetetramine (TETA), a selective Cu(II)-chelator used in the treatment of Wilson's disease, is now undergoing clinical trials for the treatment of heart failure in diabetes. Despite decades of clinical use, knowledge of its pharmacology in human subjects remains incomplete. Here, we first used liquid chromatography-mass spectrometry (LC-MS) to detect and identify major metabolites of TE...

Body fluids are a rich source of extracellular vesicles (EVs), which carry cargo derived from the secreting cells. So far, biomarkers for pathological conditions have been mainly searched from their protein, (mi)RNA, DNA and lipid cargo. Here, we explored the small molecule metabolites from urinary and platelet EVs relative to their matched source samples. As a proof-of-concept study of intra-E...

BACKGROUND We assessed the bioavailability of orange juice (poly)phenols by monitoring urinary flavanone metabolites and ring fission catabolites produced by the action of the colonic microbiota. OBJECTIVE Our objective was to identify and quantify metabolites and catabolites excreted in urine 0-24 h after the acute ingestion of a (poly)phenol-rich orange juice by 12 volunteers. DESIGN Twel...