The preparation and separation by thin-layer chromatography of the trinitrophenyl derivatives of amino acids in plasma and urine is described. The semi-quantitative procedure presents a useful addition to existing methods for screening biological fluids for aminoacidopathies.

  In this study changes in fatty acids profile during frozen storage at -18°C of Cobia (Rachycentron canadum), caught from the Persian Gulf (Bandar Abbas) were studied. Changes in saturated fatty acids (SFAs), monounsaturated fatty acids (MUFAs), polyunsaturated fatty acids (PUFAs), EPA+DHA/C16, n-3 PUFA/n-6 PUFA (n-3/n-6) and polyunsaturated fatty acids /saturated fatty acids (PUFA/SFA) were i...

A diastereoselective and enantioselective construction of 2,3-disubstituted 1,4-dicarbonyl compounds is reported. Nishiyama's RuPhebox complex (2.0 mol% catalyst loading) serves as a chiral Lewis acid in conjunction with BrCCl3 base for the oxidative homocoupling 2-acyl imidazoles via stereocontrolled reaction intermediate Ru enolates situ brominated imidazoles. Cleavage achiral imidazole auxil...

Lactobacillus strains with the potential of protecting fresh dairy products from spoilage were studied. Metabolism and antifungal activity different L. plantarum, brevis, sakei strains, isolated Sardinian meat products, assessed. The metabolite fingerprint each strain was obtained by GC-MS data submitted to multivariate statistical analysis. discriminant analysis correctly classified samples sp...

Germinated brown rice (GBR) has a high gamma-aminobutyric acid (GABA) content and antioxidant capacity; however, the effects of high-temperature cooking on these characteristics are unclear. We investigated alterations in GABA content, capacity, 15 proteinogenic amino acids, TP reducing sugar color cooked GBR at 105°C to 135°C different times (40 90 min) 105°C. The contents 9 acids decreased vi...

Carapa guianensis species belongs to the Meliaceae family and, in Brazilian Amazon, it has great importance folk medicine, mainly because oil extracted from its seeds presents varied biological activities. Thus, present work aimed analyze, by gas phase chromatography coupled mass spectrometry (GC/MS), chemical composition of manually andiroba oil, Mamangal community, located Igarapé-Miri, Pará,...

Abstract One of the main purposes for which dandelions are cultivated is to derive phenolic acids from their processing. Phenolic acids, one useful compounds in dandelion, constitute important groups therapeutically significant bioactive traditional Chinese medicine. To carry out a relatively full evaluation dandelion quality, it was found advisable use multiple indices avoid conflict with sing...

Thiamine diphosphate (ThDP) dependent enzymes are useful catalysts for asymmetric C−C bond formation through benzoin-type condensation reactions that result in α-hydroxy ketones. A wide range of aldehydes and ketones can be used as acceptor substrates; however, the donor substrate is mostly limited to achiral α-keto acids simple aldehydes. By using a unifying retro-biosynthetic approach towards...

The biochemical flexibility of the cellular translation apparatus offers, in principle, a simple route to the synthesis of drug-like modified peptides and novel biopolymers. However, only approximately 75 unnatural building blocks are known to be fully compatible with enzymatic tRNA acylation and subsequent ribosomal synthesis of modified peptides. Although the translation system can reject sub...

To enhance the production efficiency of foreign protein in baculovirus expression systems, the effects of polyhedrin fragments were investigated by fusion expressing them with the enhanced green fluorescent protein (EGFP). Recombinant viruses were generated to express EGFP fused with polyhedrin fragments based on the previously reported minimal region for self-assembly and the KRKK nuclear loca...