VDJ rearrangement and VH gene usage during fetal development in 35 outbred piglets was examined by PCR amplification of VDJs; VDJs were subsequently characterized by hybridization with VH-specific gene probes and by sequencing. VDJ rearrangement was first seen in the fetal liver on day 30 of a 114-day gestation. Four VH genes (V(H)A, V(H)B, V(H)C, and V(H)E) accounted for approximately 80% of a...

We have detected, in a CTL clone, an mRNA homologous to an immunoglobulin heavy chain variable region (VH) gene segment. A full-length copy of this mRNA was cloned and sequenced, revealing that it is the transcript of an authentic, unrearranged VH gene. The predicted protein product of this expressed VH gene is an approximately 12-kilodalton polypeptide, with secretory signal peptide leader and...

PURPOSE The condition in which visual hallucinations (VHs) are solely associated with a visual impairment is termed Charles Bonnet Syndrome (CBS). The study was undertaken to investigate whether the extent of visual acuity (VA) loss and central visual field loss predisposes a patient with age-related macular degeneration (AMD) to develop a CBS VH and, in addition, whether the progression in los...

BACKGROUND Conventional fundus imaging has been used to assess vitreous haze (VH) in patients with uveitis. Ultra-wide field (UWF) retinal imaging that uses scanning laser technology has not been evaluated for the detection of VH. This pilot study evaluates the ability of UWF imaging in detecting VH. Patients with intermediate, posterior, or panuveitis were examined to assess the level of VH us...

PURPOSE To determine if oral anticoagulation alters the association between vitreous hemorrhage (VH) and retinal tears in eyes with acute, posterior vitreous detachment (PVD). METHODS In this retrospective chart review, the complete records of consecutive patients with spontaneous, symptomatic acute PVD from a single referral-based practice were reviewed. The use of oral anticoagulants, the p...

In this paper, we first split the biharmonic equation !2u = f with nonhomogeneous essential boundary conditions into a system of two second order equations by introducing an auxiliary variable v = !u and then apply an hp-mixed discontinuous Galerkin method to the resulting system. The unknown approximation vh of v can easily be eliminated to reduce the discrete problem to a Schur complement sys...

OBJECTIVE To test the quality of postural performance in quiet upright stance in healthy young adults with vertical heterophoria (VH) within the normal range and without VH (vertical orthophoria, VO). METHODS Twenty-six subjects took part in this study. The postural stability was measured with a force platform while the subjects fixated a target at eye level in a straight ahead position, plac...

We have studied the equilibrium unfolding and the kinetics of folding and unfolding of an antibody scFv fragment devoid of cis-prolines. An anti-GCN4 scFv fragment carrying a VL lambda domain, obtained by ribosome display, served as the model system together with an engineered destabilized mutant in VH carrying the R66K exchange. Kinetic and equilibrium unfolding experiments indicate that the V...

Vestibular, oculomotor and respiratory tests were performed on 32 patients after whiplash injury caused by a rear-end car collision. Oculomotor functions were generally normal. The cervico-ocular reflex was usually absent or displayed the low gain typical of normal subjects. There was no nystagmic response to static neck torsion. The vestibulo-ocular reflex showed vestibular hyperreactivity (VH...

The functional B cell repertoire in BALB/c mice was assessed at various stages in ontogeny. This was done by analyzing VH gene family expression using the sensitive technique of in situ hybridization. The B cell repertoire was probed with the mitogen, LPS, and the antigen DNP. DNP was chosen because B cells responsive to this hapten appear very early in ontogeny. The APCs that developed after s...