Monitoring the evolution of human immunodeficiency virus type 1 (HIV-1) drug resistance requires measuring the frequency of closely related genetic variants making up the complex viral quasispecies found in vivo. In order to resolve both major and minor (>/=2%) protease gene variants differing by one or more nucleotide substitutions, we analyzed PCR products derived from plasma viral quasispeci...

background due to a recent alarming increase in the number of hiv-hcv co-infected patients in romania. objectives a cross sectional study was conducted to assess the baseline predictors of liver disease evolution. patients and methods 83 hiv-hcv co-infected patients, untreated for hcv infection, were evaluated for viral replication, liver fibrosis (estimated by a noninvasive marker - fib4), and...

conclusions the current study shows that the predominant hcv genotype among azerbaijani patients with established chronic hepatitis c is subtype 1b (71.1%) followed by subtype 3a (17.0%). results hcv genotyping of positive plasma samples demonstrated that predominant hcv subtype was noted for 1b (71.1%) followed by subtype 3a (17.0%), genotype 2 (6.8%), 1a (1.7%), and mixed infection (3.4%). th...

The question of a possible relationship between the RNA content of poliovirus and its reactivity as Nor H-antigen was examined with double-labeled virus preparations (32P-RNA, 14C-Protein). The following results were obtained: 1. Complete virus particles containing RNA may be Nor H-reactive. 2. Heat inactivation of poliovirus causes dissociation of capsid and RNA. 3. Under certain experimental ...

The microbial inactivation/sterilization is an important part of our daily healthy life. This paper presents the overview on the sterilization process. Comparing the effectiveness of the sterilization agents it has been found that the short High Electric Field [HEF] pulse is the most efficient inactivation agent. This HEF pulse creates pores on the cell surface and kills the cell. Electroporati...

background and aims: genotyping assay has been accepted as a guidance in the therapeutic management of human immunodeficiency virus 1 (hiv-1). but, it is not commonly used in our country due to its high running cost. the aim of this study is evaluate an in-house genotyping resistance test (grt) for hiv-1. methods: hiv-1 rna of 20 samples were extracted from plasma and rt nested- pcr was perform...

objective: hepatitis c virus is the major cause of viral hepatitis and its diagnosis in suspected specimens is of great importance. the risk of transfusion- transmitted virus infection is primarily the result of failure in serological screening tests to detect recently infected donors in the pre-seroconversion window period of infection. therefore, sensitive and accurate diagnosis of hcv prior ...

conclusions this study showed that cccdna can be detected in the plasma specimen of 17.9% of iranian treatment-naive patients with chronic hepatitis b infection. therefore, designing prospective studies focusing on the detection of cccdna in these patients would provide more information. results the mean (sd) age of the patients was 41.1 ± 12.4 years (range, 20 - 62 years). from a total of 106 ...