Objective(s): Juglone is isolated from many species of the Juglandaceae family and used as an anti-viral, anti-bacterial, and anti-tumor therapeutic. Here, we evaluated juglone-induced antitumor effect in ovarian cancer SKOV3 cells. Materials and Methods: MTT assay was performed to examine juglone anti-proliferative effect. Cell cycle and apoptosis were studied using flow cytometry in juglone-t...

Nucleocapsid (N) protein of infectious bronchitis virus (IBV), one of the viral structural proteins, inducesstrong antibody response in natural infection. In this study, a simple, recombinant N protein-based dot-blottest was developed to serologically examine chicken serum samples for the presence of IBV antibody.Initially, 72 serum samples were tested for the presence of IBV antibody using a c...

Infectious bursal disease (IBD) is an economically important viral disease of chickens with worldwide distribution which suppresses the immune system of young chickens. VP2 is the major host-protective protein of infectious bursal disease virus (IBDV). The encoding region of VP2 protein was PCR amplified from a plasmid containing a cDNA fragment of large genomic segment of IBDV, strain D78. Thi...

Abstract Approaches are needed for therapy of the severe acute respiratory syndrome from SARS-CoV-2 coronavirus (COVID-19). Interfering with interaction viral antigens angiotensin converting enzyme 2 (ACE-2) receptor is a promising strategy by blocking infection coronaviruses into human cells. We have implemented novel protein engineering technology to produce super-potent tetravalent form ACE2...

conclusions igm alone is not a marker for viral shedding in genital tract. molecular testing in conjunction of igg test should be evaluated as an option to determine hsv status, and applied for research on hsv genital infections records. methods two hundred and eight females were included in the study; igm antibodies against hsv1/2 were detected in serum samples; the real-time polymerase chain ...

Background:Influenza virus is globally pathogenic and it is usually associated with zoonotic respiratory diseases. This virus has caused a number of pandemics with a high mortality rate. The non-structural (NS1) protein of influenza A viruses is a non-essential virulence factor that has multiple accessory functions during viral infection. This protein is highly conservative. It has been shown t...

Geminivirus replication initiator protein (Rep) is a multifunctional viral required for replication. During the process of replication, Rep acts as site- and strand-specific endonuclease, ligase, ATPase, helicase. B' motif β-hairpin loop geminivirus are conserved important Rep-mediated helicase activity To dissect roles various amino acid residues in its unwinding DNA, we investigated eight nea...

The occurrence of the novel severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), responsible for coronavirus disease 2019 (COVD-19), represents a catastrophic threat to global health. Protruding from viral surface is densely glycosylated spike (S) protein, which engages angiotensin-converting enzyme 2 (ACE2) mediate host cell entry. However, studies have reported susceptibility in intr...

Protein-protein interactions play a key role during each stage of the viral life cycle. Thus, inhibiting viral protein-protein interactions with the peptides derived from the binding interfaces is a promising approach for potential multi-epitope based treatments. However, molecular mechanisms underlying these protein interactions are not yet fully understood. This study is focused on a comprehe...