Chromatin immunoprecipitation coupled with DNA sequencing (ChIP-seq) is the major contemporary method for mapping in vivo protein-DNA interactions in the genome. It identifies sites of transcription factor, cofactor and RNA polymerase occupancy, as well as the distribution of histone marks. Consortia such as the ENCyclopedia Of DNA Elements (ENCODE) have produced large datasets using manual pro...

Chromatin immunoprecipitation and sequencing (ChIP-seq) provides a static snap-shot of DNA-associated proteins which fails to reflect the dynamics of the DNA-bound proteome. Now, Catic and co-workers combine ubiquitin ChIP-seq and proteasome inhibitors to map sites of DNA-associated protein degradation on a genome-wide scale. They identify an ubiquitin ligase which targets a transcriptional rep...

Chromatin-immunoprecipitation (ChIP) employs generally a mild formaldehyde cross-linking step, which is followed by isolation of specific protein-DNA complexes and subsequent PCR testing, to analyze DNA-protein interactions. Poly(ADP-ribosyl)ation, a posttranslational modification involved in diverse cellular functions like repair, replication, transcription, and cell death regulation, is most ...

Cross-talk between DNA methylation and histone modifications drives the establishment of composite epigenetic signatures and is traditionally studied using correlative rather than direct approaches. Here, we present sequential ChIP-bisulfite-sequencing (ChIP-BS-seq) as an approach to quantitatively assess DNA methylation patterns associated with chromatin modifications or chromatin-associated f...

There is hardly a more important sensory modality for humans and other mammals than vision. The first and best-known part of the visual system is the retina, which is not a mere photoreceptor or static camera but a sophisticated feature preprocessor with continuous input and several parallel output channels. These interacting channels represent the visual scene. Never before has it been known i...

Automated visual inspection, a crucial manufacturing step, has been replacing the more time-consuming and less accurate human inspection. This research explores automated visual inspection of surface defects in a light-emitting diode (LED) chip. Commonly found on chip surface are water-spot defects which impair the appearance and functionality of LEDs. Automated inspection of water-spot defects...

In this paper, an imaging chip for acquiring range information using by 0.35 μm CMOS technology and 5V power supply has been described. The system can extract range information without any mechanical movement and all the signal processing is done on the chip. All of the image sensors and mixed-signal processors are integrated in the chip. The design range is 1.5m-10m with 18 scales.

Automated visual inspection, a crucial manufacturing step, has been replacing the more time-consuming and less accurate human inspection. This research explores automated visual inspection of surface defects in a light-emitting diode (LED) chip. Commonly found on chip surface are water-spot blemishes which impair the appearance and functionality of LEDs. Automated inspection of water-spot defec...

We have micromachined a silicon-chip device that transports DNA with a Brownian ratchet that rectifies the Brownian motion of microscopic particles. Transport properties for a DNA 50-mer agree with theoretical predictions, and the DNA diffusion constant agrees with previous experiments. This type of micromachine could provide a generic pump or separation component for DNA or other charged speci...