Background: The cryopreservation of immature oocytes at the germinal vesicle (GV) stage would create an easily accessible, nonseasonal source of female gametes for research and reproduction. The present study investigated the ability of ovine oocytes vitrified at the GV stage using a cryoloop to be subsequently matured, fertilized and cultured in vitro to blastocyst-stage embryos. Materials and...

This article aims to report successful live births after transfer of fresh blastocyst or vitrified/warmed blastocyst derived from intracytoplasmic sperm injection (ICSI) on day-1 of unfertilized mature eggs (so-called "rescue ICSI") with spindle examination using polarized light microscopy. Two couples who had rescue ICSI performed achieved a positive pregnancy result after the transfer of a fr...

BACKGROUND The Rapid-i is a new FDA cleared closed carrier for embryo vitrification. The cooling rate of - 1220°C/min is far lower than that reported with open vitrification systems such as the cryoloop (-15,000°C/min). Little published data is currently available on this device. This study presents our initial clinical data, as well as live birth outcomes, with the Rapid-i. The efficacy of thi...

Background: The aim of this study was to investigate the compare of Conventional (CV) and Direct Cover (DCV) vitrification and effect of sodium selenite(SS) that supplemented with FBS and BSA on reactive oxygen species (ROS) production, total antioxidant capacity (TAC) and glutathione peroxide (GPx) activity of cultured pre-antral follicles derived from vitrified and non-vitrified ovarian tissu...

Background: micro-testicular sperm extraction (TESE) has been the first choice for Nonobstructive azoospermia (NOA) patients its higher retrieval rate under microscope, meanwhile, or oocyte cryopreservation are widely applied in in-vitro fertilization (IVF) treatment. But few researches have systematically explored effect of and one study. Methods: we retrospectively collected analyzed data pre...

The aim of this study was to compare in vitro survival rates of in vivo and in vitro-produced bovine embryos by slow freezing or solid surface vitrification. In vivo-produced blastocysts (n = 210) and in vitroproduced blastocysts (n = 445) were randomly allocated in two cryopreservation groups. Group 1 embryos were exposed to 1.5 M ethylene glycol (EG) for 5 min, loaded in 0.5 ml straws, frozen...

The aim of this study was to evaluate the suitability of a commercial kit for bovine embryo vitrification for cryopreserving cat oocytes and to evaluate comparatively the effects of its use with slow freezing procedure on cryotolerance in terms of morphology and oocyte resumption of meiosis. Germinal vesicle stage oocytes isolated from cat ovaries were either vitrified (n = 72) using a vitrific...

This study was designed to compare the efficiency of the Cryotop and Calibrated plastic inoculation loop (CPIL) devices for vitrification of rabbit embryos on in vitro development and implantation rate, offspring rate at birth and embryonic and fetal losses. CPIL is a simple tool used mainly by microbiologists to retrieve an inoculum from a culture of microorganisms. In experiment 1, embryos we...

Assisted reproductive technologies such as artificial insemination (AI) and multiple ovulation and embryo transfer (MOET) have been introduced to overcome reproductive inefficiencies and accelerate genetic gain. However, MOET in water buffalo is compromised by the poor ovulatory response of this animal species to hormonal treatment (Karaivanov 1986; Karaivanov et al. 1990; Misra et al. 1990; Cr...

Background: Oocyte vitrification and assisted oocyte activation have increasingly important implications in assisted reproductive technology. However, an important area of concern with matured oocyte cryobiology is that elements of oocytes intimately involved in metaphase-II arrest may be modified by cryopreservation. In a comparison between different cellular characteristics of unvitrified, vi...