Wheat, rice, and corn are the most widely eaten whole grains, followed by oats, rye, and barley. A wheat kernel consists of 80–85% endosperm, 10–14% bran, and 2.5–3% germ (Figure 1). Whole grains contain a high amount of bioactive substances such as fiber, vitamins, minerals, antioxidants, and other phytochemicals such as betaine, choline, sulfur amino acids, and melatonin, which account for at...

The objective of this study was to evaluate the feasibility of an aqueous process to extract the residual oil from sunflower press cakes using a co-rotating twin-screw extruder. Two different configurations were tested: the expression fromwhole seeds followed by the aqueous extraction, in two successive apparatus or in the same one. For the aqueous extraction stage, the oil yield depended on th...

Fast pyrolysis bio-oils are feasible energy carriers and a potential source of chemicals. Detailed characterization of bio-oils is essential to further develop its potential use. In this study, quantitative 13C nuclear magnetic resonance (13C NMR) combined with comprehensive two-dimensional gas chromatography (GC × GC) was used to characterize fast pyrolysis bio-oils originated from pinewood, w...

The influence of various oil cakes has been investigated for high level production of lipase using Aspergillus tamarii MTCC 5152. By solid state fermentation in wheat bran containing 2.5% w/w gingili oil cake at 70% v/w moisture content the fungus produced a maximal yield of lipase (758 ± 3.61 u/g) after 5 days of incubation using 2% v/w inoculum containing 10(6) spores/mL. Wheat bran and gingi...

The initial step in the conjugation of ubiquitin to substrate proteins involves the activation of ubiquitin by ubiquitin activating enzyme, E1. Previously, we purified and characterized multiple species of E1 from wheat germ. We now describe the isolation and characterization of a cDNA clone encoding E1 from wheat. This clone (UBA1) was isolated from a cDNA expression library with anti-wheat E1...

Several yeasts and yeast products were tested in adult diets for the medfly Ceratitis capitata (Wiedemann), oriental fruit fly Bactrocera dorsalis (Hendel), and melon fly, Bactrocera curcurbitae (Coquillett) (Diptera: Tephritidae) and in larval liquid diet for mass-rearing B. dorsalis. Three hydrolyzed brewer’s yeasts (FNILS65, FNI200 and FNI210), one glutamine enriched yeast (GSH), one vitamin...

The glycoproteins of plasma membranes from axenically grown Dictyostelium discoideum and human red blood cells (O+) were characterized according to their apparent molecular weights in sodium dodecyl sulfate-polyacrylamide gels and their ability to bind lectins. This was achieved by diffusing each of several fluorescein-conjugated lectins into sodium dodecyl sulfate-polyacrylamide gels which con...

BACKGROUND Bioethanol can be produced from sugar-rich, starch-rich (first generation; 1G) or lignocellulosic (second generation; 2G) raw materials. Integration of 2G ethanol with 1G could facilitate the introduction of the 2G technology. The capital cost per ton of fuel produced would be diminished and better utilization of the biomass can be achieved. It would, furthermore, decrease the energy...

of human erythrocyte ghosts were solubilized with 0.5yc Triton X-100 in 56 mM sodium borate, pH 8.0. This procedure solubilized 51% of the membrane protein, 81% of the sialic acid, 89% of the receptors for the Agaricus bisporus lectin and 70 to 75% of the wheat germ agglutinin and Ricinus communis lectin receptors. The solubilized glycoproteins were then separated by affinity chromatography on ...