The effects of osmoprotectants (such as glycine betaine and proline) and particulate materials on the fermentation of very high concentrations of glucose by the brewing strain Saccharomyces cerevisiae (uvarum) NCYC 1324 were studied. The yeast growing at 20 degrees C consumed only 15 g of the sugar per 100 ml from a minimal medium which initially contained 35% (wt/vol) glucose. Supplementing th...

Proline accumulation in NaCl-treated excised barley (Hordeum vulgare var Larker) leaves was studied. Leaves were treated by placing the cut end in NaCl solutions and allowing the salt to enter the leaf via the transpiration stream. Leaves treated this way maintained turgor while the sodium content increased and the osmotic potential decreased. Proline began accumulating after 12 hours and conti...

Food-derived bioactive peptides are reported to express a variety of functions in vivo. We studied the in vitro effect of three bioactive tripeptides, isoleucine-proline-proline (IPP), valine-proline-proline (VPP) and leucine-lysine-proline (LKP), on osteoblast proliferation and gene expression. We used UMR-106 osteosarcoma cells, human marrow-derived mesenchymal stem cells (hMSC) and osteoblas...

BACKGROUND Transforming growth factor-beta(1) (TGF-beta(1)) contributes to arterial remodeling by stimulating vascular smooth muscle cell (VSMC) growth and collagen synthesis at sites of vascular injury. Because L-arginine is metabolized to growth-stimulatory polyamines and to the essential collagen precursor L-proline, we examined whether TGF-beta(1) regulates the transcellular transport and m...

To investigate the effects of saline stress on seed germination, ion distribution, and organic solutes changes of sunflower (Helianthus annuus L.), in the present study, seeds and 3-week-old seedlings were subjected to a series of external NaCl concentrations (5–200 mmol). The results showed that high salinity (200 mmol) remarkably inhibited the germination of seed and delayed germination time ...

Aim. To analyze the effect of L-proline accumulation on chlorophyll content in transgenic wheat plants with a double-stranded RNA suppressor proline dehydrogenase gene under physiological and stress conditions. Methods. Agrobacterium-mediated transformation planta; biochemical determination free content; spectrophotometric photosynthetic pigments mathematical statistics. Results. It was shown t...

Abstract Global climate change induced environmental stress such as salinity significantly impact growth and crop productivity. Red amaranth ( Amaranthus tricolor L.) is a vegetable commodity that has high nutritional value, but its productivity will decrease when grown in saline area. Seed priming one of the pre-planting treatment techniques used to increase plant under unfavourable conditions...

Proline accumulation was often correlated with drought tolerance of plants infected by arbuscular mycorrhizal fungi (AMF), whereas lower proline in some AM plants including citrus was also found under drought stress and the relevant mechanisms have not been fully elaborated. In this study proline accumulation and activity of key enzymes relative to proline biosynthesis (▵(1)-pyrroline-5-carboxy...

Staphylococcus aureus can grow virtually anywhere in the human body but needs to import proline through low- and high-affinity proline transporters to survive. This study examined the regulation of the S. aureus putP gene, which encodes a high-affinity proline permease. putP::lacZ and putP::lux transcriptional fusions were constructed and integrated into the genomes of several S. aureus strains...

The R3230AC mammary carcinoma grows faster in diabetic rats; tumor cells from diabetic rats demonstrate increased proline transport and incorporation into pro teins compared to tumor cells obtained from intact rats. Tumor cells from insulin-treated diabetic rats showed a decreased rate of proline transport and a lower amount of proline incorporation, both of which were comparable to that in cel...