Withania somnifera is a medicinal plant extends over a large area, from the Atlantic ocean to South East Asia and from the Mediterranean region to South Africa. The medicinal plants are widely used by the traditional medical practitioners for curing various diseases in their day to day practice. In traditional systems of medicine, different parts (leaves, stem, flower, root, seeds, bark and eve...

OBJECTIVE Oxygen free radicals may be implicated in the pathogenesis of ischemia reperfusion damage. The beneficial effects of antioxidant nutrients, as well as complex plant extracts, on cerebral ischemia-reperfusion injuries are well known. This study was conducted to determine the effects of the hydro-alcoholic root extract of Withania coagulans on CA1 hippocampus oxidative damages following...

Withania somnifera (Ashawagandha) is very revered herb of the Indian Ayurvedic system of medicine as a Rasayana (tonic). It is used for various kinds of disease processes and specially as a nervine tonic. Considering these facts many scientific studies were carried out and its adaptogenic / anti-stress activities were studied in detail. In experimental models it increases the stamina of rats du...

“Withanolides”, the legendary bioactive metabolite present in leaves and roots of Withania somnifera, is well known for its therapeutic activities since ancient times. Several efforts have been made to produce these metabolites under in vitro condition. In the present study, we have tested the effects of exogenous supplementation of IBA and IAA in W. somnifera in vitro root cultures with the ai...

Roots of Withania somnifera are widely used in Indian herbal formulations. Withaferine A and withanolide A are two pharmacologically important withanolides present in the roots of the plant. HPLC method using DAD and ELSD detector was developed for simultaneous determination of withaferine A and withanolide A from the roots and herbal formulation of the plant. Coarse powder of root was extracte...

In vitro aqueous and ethanol extracts of Withania somnifera (leaves and fruits) and Curcuma Longa were evaluated for antibacterial activities against Basillus subtillis, Staphylococcus aureus, Escherichia coli and S.epidermitis. The inorganic extract of W. somnifera leaves showed more antibacterial activity as compared to the organic fraction. While both the organic and inorganic phases of frui...

In the present study, we have established a stable transformation protocol via Agrobacterium tumafacines for the pharmaceutically important Withania somnifera. Six day-old nodal explants were used for 3 day co-cultivation with Agrobacterium tumefaciens strain LBA4404 harbouring the vector pCAMIBA2301. Among the different injury treatments, sonication, vacuum infiltration and their combination t...

AIM The aim was to investigate antibacterial activity of Withania somnifera (Ashwagandha), an Indian traditional medicinal plant against Escherichia coli O78, a pathogenic strain. MATERIALS AND METHODS Two-fold serial dilutions of 20% aqueous W. somnifera root (WSR) extract were inoculated with E. coli O78 @ 1*10(7) colony forming units grown in nutrient broth. Following inoculation, turbidit...

Few anti-pigmenting agents have been designed and developed according to their known hyperpigmentation mechanisms and corresponding intracellular signaling cascades. Most anti-pigmenting agents developed so far are mechanistically involved in the interruption of constitutional melanogenic mechanisms by which skin color is maintained at a normal and unstimulated level. Thus, owing to the difficu...