The experiment was conducted to determine the effect of microbial phytase in Ca deficient and available aP diets on broiler performance and some blood traits. Three hundred day-old chicks (Ross 308) were distributed into five equal groups with two replicates. The control diets (T1) contained Corn-SBM, 0.9% Ca and 0.40% aP without enzyme, whereas T2, T3, T4 and T5 contained Corn-SBM, 0.60% Ca an...

Two experiments were conducted to examine the influence of addition of microbial phytase to diets differing in native phytase content, on the performance of sows and digestibility of nutrients. Barley and wheat grains relatively poor in native phytase used in Experiment 1 mixtures were replaced by triticale and rye (rich in this enzyme) in Experiment 2 diets. There were 3 groups in each experim...

An experiment was conducted to determine effect of supplementing maize based diet with different levels of microbial phytase on apparent nutrient bioavailability and tibia bone mineral of broilers. Eighty, day-old broiler were randomly allotted to five dietary treatments. The control diet contained 0 FTU/kg microbial phytase which was replaced by 200, 400, 600 and 800 FTU/kg microbial phytase o...

Several Bacillus strains belonging to the B. subtilis/amyloliquefaciens group isolated from plant-pathogen-infested soil possess plant-growth-promoting activity [Krebs, B. et al. (1998) J Plant Dis Prot 105, 181-197]. Three out of the four strains investigated were identified as B. amyloliquefaciens and were able to degrade extracellular phytate (myo-inositol hexakisphosphate). The highest extr...

The degradation of phytin in germinating mung bean seeds has been found to be associated with the increased activity of phytase in the cotyledon. In the differentiated embryo the increase of this activity is very low all throughout the growth periods studied. Phytase appears in the cotyledon during germination. No activity has been detected in the cotyledons of unsoaked seeds. Cycloheximide (10...

The present article implies an extracellular phytase production by Aspergillus niger under submerged and solid fermentation. Physical and Chemical conditions tested for optimal production of phytase using the single variable mode optimization technique. A considerable higher phytase production was obtained using rice bran as solid substrate (17.8 U/ml & 28.6 U/ml) at pH 5, on 4 day of incubatio...

Five Yersinia strains, biochemically mimicking Yersinia intermedia, were isolated from patients with acute diarrheal diseases seen at the University of Lagos (Nigeria) Teaching Hospital between October 1979 and September 1982. The five isolates utilized citrate as their source of carbon and fermented sucrose, L-rhamnose, and alpha-methyl-D-glucoside, but unlike any other Y. intermedia biogroup,...

This experiment was conducted to investigate the effect of phytase supplementation in diets reduced amino acids ( AA ), crude protein CP and phosphorus P ) on growth performance, carcass traits, tibia ash broiler chickens. A total 2,240 unsexed Ross 308 chickens were used 56 floor pens with 40 birds each fed one eight dietary treatments seven replicates until 35 d age. positive control PC diet ...

A phytase was isolated and partially purified from the pollen of Lilium longiflorum Thumb. Optimum activity was at pH 8.0. The phytase was activated by Ca(2+) and Sr(2+) but not by the other divalent cations tested. Activity was inhibited by ethylenediaminetetraacetate. The phytase had a temperature optimum of 55 to 60 degrees C and an activation energy of about 12,700 calories/mole. Extraction...

Using statistical optimization, we enhanced the activity of phytase by a new Saccharomyces cerevisiae strain cultured in mineral medium. Concentrations of carbon source and inducer of phytase production were optimized using a 2(2) full factorial CCD and response surface methodology (RSM). Urea was fixed as nitrogen source in culture medium (0.15%, w/v). The culture medium consisting of 2.5% suc...