To maintain genomic stability, despite constant exposure to agents that damage DNA, eukaryotic cells have developed elaborate and highly conserved pathways of DNA damage sensing, signalling and repair. In this review, we concentrate mainly on what we know about DNA damage sensing with particular reference to Lcd1p, a yeast protein that functions early in DNA damage signalling, and MDC1 (mediato...

lipophilic yeast of the genus malassezia (pityrosporum) belongs to the normal flora of human skin and many warm-blooded animals. these fungi can produce a diverse range of diseases that the best known and most frequent of them is pityriasis versicolor, a chronic recurrent skin disease occurring primarily in tropical regions. the genus malassezia has been recently enlarged to include seven disti...

there is a great call for using microbial bio-decaffeination approach to remove caffeinefrom caffeinated products and industrial wastes. we aimed in this study to screen strainsof yeasts which exhibit high caffeine tolerance and to investigate the bio-degradation ofcaffeine under growth conditions. sixteen yeast strains were isolated from the cultivatedtea soils collected from sites of northern...

To produce chitoorigosaccharides using chitosan, we attempted to construct Paenibacillus fukuinensis chitosanase-displaying yeast cells as a whole-cell biocatalyst through yeast cell-surface engineering. The localization of the chitosanase on the yeast cell surface was confirmed by immunofluorescence labeling of cells. The chitosanase activity of the constructed yeast was investigated by halo a...

Recent results point to an important role for the nucleolus in the senescence of yeast cells. A further report suggests that the formation and preferential accumulation in mother cells of extrachromosomal rDNA circles is a cause of aging in yeast; this may be an ancient and conserved mechanism of senescence.

The hPMS2 mutation E705K is associated with Turcot syndrome. To elucidate the pathogenesis of hPMS2-E705K, we modeled this mutation in yeast and characterized its expression and effects on mutation avoidance in mammalian cells. We found that while hPMS2-E705K (pms1-E738K in yeast) did not significantly affect hPMS2 (Pms1p in yeast) stability or interaction with MLH1, it could not complement the...

conclusions direct sample pcr has the potential to rapidly and accurately diagnose candida vulvovaginitis in patients, especially if sufficient samples are obtained. results the results showed that out of the 150 samples, 55 were positive and 63 samples were negative by both methods, and 32 samples were positive using the culture method, but negative by ds-pcr. all positive ds-pcr samples had >...

This paper proposes a novel pixel interval down-sampling network (PID-Net) for dense tiny object (yeast cells) counting tasks with higher accuracy. The PID-Net is an end-to-end convolutional neural (CNN) model encoder–decoder architecture. operations are concatenated max-pooling to combine the sparse and features. addresses limitation of contour conglutination objects while counting. evaluation...

A single species, Candida albicans, causes half of all invasive fungal infections in humans. Unlike other fungal pathogens, this organism switches between growth as budding yeast and as pseudohyphal and hyphal filaments in host organs and in vitro. Both cell types play a role in invasive disease: while hyphal and pseudohyphal filaments penetrate host cells and tissues, yeast cells are likely to...

Paracoccidioides brasiliensis, a thermal dimorphic fungal pathogen, produces a melanin-like pigment in vitro and in vivo. We investigated the involvement of carbohydrates and monoclonal antibody to CD18, on phagocytosis inhibition, involving macrophage receptors and the resistance of melanized fungal cells to chemically generated nitric oxide (NO), reactive oxygen species (ROS), hypochlorite an...