Model-based design of biological parts is a critical goal of synthetic biology, especially for eukaryotes. Here we demonstrate that nucleosome architecture can have a role in defining yeast promoter activity and utilize a computationally-guided approach that can enable both the redesign of endogenous promoter sequences and the de novo design of synthetic promoters. Initially, we use our approac...

The expression of mumps virus nucleocapsid protein in yeast Pichia pastoris was investigated. Viral nucleocapsid proteins usually elicit a strong long-term humoral immune response in patients and experimental animals. Therefore, the detection of antibodies specific to mumps virus nucleoprotein can play an important role in immunoassays for mumps diagnosis. For producing a high-level of recombin...

A feeding trial with twenty-seven male weaned Zandi lambs (initial body weight 27.1±0.38 kg) was conducted to evaluate the effect of different levels of Saccharomyces cerevisiae SC47 in diet containing high concentrate (85%) on the growth performance, blood parameters and immune system status. Lambs were allocated to one of three treatment diets in a completely randomized design with 3 replicat...

The yeast two-hybrid system is a powerful experimental approach for the characterization of protein/ protein interactions. A unique strength of the yeast two-hybrid system is the provision for genetic selection techniques that enable the identification of specific protein/protein interactions. We now report the development of a modified yeast two-hybrid system which enables genetic selection ag...

The current epidemic of diabetes indicates the need of proper and effective medications that are limited in their potency to have many side effects. Thus, introduction of alternative and complementary medicine is now in picture. The main objective of this study was to analyze the rate of glucose transport across cell membrane in yeast cells system in the presence of the ethanolic extract of Bru...

chaperones are produced by prokaryotic, yeast and higher eukaryotic cells for various purposes. over-expression of each chaperone or sets of them affect the production level of a recombinant protein in the cell. on the basis of this hypothesis, five different plasmids with 5 different combinations of 6 chaperones molecule, transformed into escherichia coli along with human basic fibroblast grow...

Intermolecular interactions of Tula hantavirus N (nucleocapsid) protein were detected in the yeast two-hybrid system, prompting further attempts to study this phenomenon. Using chemical cross-linking and immunoblotting it was shown that the N protein from purified virus and from infected cell lysates as well as recombinant protein produced in a baculovirus expression system are capable of formi...

Rice seed expression (cDNA) library in the Lambda Zap 11® phage constructed from the developing grain 10-20 days after flowering was transformed into yeast for functional complementation assays in three salt sensitive yeast mutants S. cerevisiae strain CY162, G19 and Axt3K. Transformed cells of G19 and Axt3K with pYES vector with cDNA inserts showed enhance tolerance than those with empty pYes ...

In the last decade, the budding yeast Saccharomyces cerevisiae has been used as a model system to study the mechanisms of the human aging process and of age-associated neurodegenerative disorders such as Parkinson's, Huntington's, Alzheimer's, and amyotrophic lateral sclerosis. S. cerevisiae is a facultative aerobic, unicellular yeast, and despite their simplicity, yeast cells possess most of t...

objective(s) to compare two identification methods, i.e., restriction fragment length polymorphism (rflp)-pcr analysis and enzymatic method rapid tm yeast plus system to identify different species causing vulvovaginal candidiasis (vvc). materials and methods vaginal discharges of women who had attended the gynecology outpatient clinic of mobini hospital in sabzevar, iran were collected using co...