Vincristine (VCR) has been shown to enhance the trans port of methotrexate (MTX) in animal systems and in acute myelogenous leukemia cells in vitro. In this report, the uptake of MTX and the effect of VCR were studied in two human lymphoblastoid cell lines grown in tissue culture. The effect of VCR on MTX suppression of deoxyuridine incorporation into DNA by these cell lines was also investi ga...

Barrett's esophagus is a premalignant condition arising in response to chronic reflux esophagitis. Inducible nitric oxide synthase (¡NOS;NOS-2) and cyclooxygenase-2 (COX-2) are mediators of inflammation and regu lators of epithelial cell growth. Expression levels of ¡NOSand COX-2 are high in colorectal adenomas and carcinomas, and COX-2 expression is elevated in gastric cancers. To determine ...

Genomic DNA was extracted from archival pathology specimens comprising 10 squamous and 14 adenocarcinomas, including 7 with Barrett's epithelium adjacent to tumor, and corresponding normal esoph agus from the resection margin. The polymerase chain reaction was used to amplify selected exons ofp53 which were analyzed for mutations using single-strand conformation polymorphism analysis. Mutations...

The hypothesis that the naturally occurring analgesic peptide, â-endorphin, is released in the brain in response to pain had never been directly validated. In this study, we applied a brain microdialysis method for monitoring â-endorphin release in vivo, to test this hypothesis in the brains of conscious, freely moving rats. Herein we first show that endogenous â-endorphin can be measured in vi...

The catabolism <>l5-fluorouracM ill mi »as measured in isolated perfused rat liver (IPRL) at various times of the day. IPRLs were prepared from rats sacrificed at 3-h intervals and the elimination rate of FUra and FUra catabolites (i.e., rate leaving the II'KI in the effluent perfusate) following infusion of |'I l|l I ni »as analy/.ed for circadian periodicity. Animals were housed under stand...

C10H24F6N6O8SÌ, tetragonal, PA /̂n (no. 86), a = 15.646(1) Â, c = 8.2024(5) Â, V = 2007.9 Â, Ζ = 4, Rgl(F) = 0.052, wRrefiF) = 0.117, T= 233 K. Source of material The racemic title compound was unexpectedly obtained from a mixture of 2-azido-l,3-dimethoxyimidazolium hexafluorophosphate [1], dimethyl acetylenedicarboxylate and copper wire in methanol. Suitable crystals were harvested after allowi...

Pandemi Covid-19 yang terjadi di berbagai belahan dunia sangat berdampak pada kerja dokter dan tenaga kesehatan lainnya. Kasus aktif terus merangkak naik mengakibatkan para terlibat langsung menangani pasien beresiko mengalami stress saat bekerja. Stress dirasakan diakibatkan oleh banyak faktor internal eksternal. Tujuan tinjauan sistematis adalah mengidentifikasi faktor-faktor penyebab usaha d...