نتایج جستجو برای: آنزیم hrp
تعداد نتایج: 14398 فیلتر نتایج به سال:
In this article, the authors present a conceptual framework named adaptive seriational risk parity (ASRP) to extend hierarchical parity (HRP) as an asset allocation heuristic. The first step of HRP (quasi-diagonalization), determining hierarchy assets, is required for actual done in second (recursive bisectioning). original scheme, found using single-linkage clustering correlation matrix...
Resonance Raman spectra of horseradish peroxidase (HRP) in the 200 to 600 cm" region were measured for the reduced and oxidized states and in the presence and absence of substrate, For the reconstituted HRP with a S4Fe-substituted heme, the Raman line of ferro HRP at 244 cm" was shifted to 246 cm" but all other modes remained unshifted. The amount of the frequency shift agreed with that observe...
Tight junctions between bovine brain capillary endothelial cells in primary cell culture are impermeable to horseradish peroxidase (HRP) after 5 min of incubation. However, following 1-5 min of exposure to 1.6 M arabinose, HRP penetrates the extracellular space between successive tight junctions. Endothelial cells in control cultures contain a small number of cytoplasmic pits and vesicles conta...
Background and Objectives: Quality of work life is an increasingly important organizational factor in health facilities. Most studies on quality of work life in hospitals have been conducted in developed countries. The few studies performed in developing countries have targeted low- and middle-performance hospitals, and they have not explored the relationship between quality of work life and...
BACKGROUND The purpose of this study was to devise a novel electrochemical immunosensor for ultrasensitive detection of alfa-fetoprotein based on Fe(3)O(4)/Au nanoparticles as a carrier using a multienzyme amplification strategy. METHODS AND RESULTS Greatly enhanced sensitivity was achieved using bioconjugates containing horseradish peroxidase (HRP) and a secondary antibody (Ab(2)) linked to ...
The plant pathogenic bacterium Pseudomonas syringae is divided into pathovars differing in host specificity, with P. syringae pv. syringae (Psy) and P. syringae pv. tomato (Pto) representing particularly divergent pathovars. P. syringae hrp/hrc genes encode a type III protein secretion system that appears to translocate Avr and Hop effector proteins into plant cells. DNA sequence analysis of th...
To overcome the sensitivity limit for small molecules (haptens) in immunoassays based on antigen-horseradish peroxidase (HRP) conjugates as labels, a novel approach was established that afforded very low detection limits. Biotinylated anti-HRP antibody was utilized in order to attach, via a streptavidin bridge, liposomaly entrapped HRP. Fentanyl, used as a model antigen, could be determined via...
Pregnant women have an increased susceptibility to infection by Plasmodium falciparum. Parasites may be present in the placenta yet not detectable in peripheral blood smears by routine light microscopy. In order to determine how frequently misdiagnosis occurs, peripheral blood and placental samples were collected from 1,077 Cameroonian women at the time of giving birth and examined for the pres...
We have studied the direct electrochemistry of horseradish peroxidase (HRP) coupled to gold nanoparticles (AuNP) using electrochemical techniques, which provide some insight in the application of biosensors as tools for diagnostics because HRP is widely used in clinical diagnostics kits. AuNP capped with (i) glutathione and (ii) lipoic acid was covalently linked to HRP. The immobilized HRP/AuNP...
Horseradish peroxidase (HRP) and thionine (TN) were co-adsorbed onto a porous carbon felt (CF), and the resulting HRP and TN-adsorbed CF (HRP-TN-CF) was successfully used as a working electrode unit of a novel bioelectrocatalytic flow detector for a highly sensitive amperometric determination of hydrogen peroxide (H(2)O(2)). Co-adsorbed TN was essential to enhance the cathodic peak current of H...
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