Intrahepatic cholangiocarcinoma (IHC) is a biliary tumor that arises from the intrahepatic bile ducts. It is the second most common primary hepatic malignancy after hepatocellular carcinoma (HCC), with higher prevalence in patients with cirrhosis and Hepatitis B and C. On imaging, IHC typically demonstrates progressive centripetal progression and significant delayed enhancement with dilatation ...

UNLABELLED Immunohistochemistry (IHC) has been employed in the differential diagnosis of tumors. OBJECTIVE To assess the use of IHC in cases of head and neck tumor. METHOD This is a retrospective study of the cases included in the Cancer Registry of the institution. RESULTS IHC was used in 76 (11%) of 704 pathology tests. Most cases were carcinomas (85.80%), and 83.66% of them were squamo...

F or decades anatomical pathologists have established and managed diagnostic immunohistochemistry (IHC) laboratories to assist in accurate tissue diagnosis. Although test validation, quality control (QC), and quality assurance (QA) procedures have always been a part of routine operations, test results were always viewed in the context of clinical, morphological, and other IHC test results. Howe...

INTRODUCTION While some targeted agents should not be used in squamous cell carcinomas (SCCs), other agents might preferably target SCCs. In a previous microarray study, one of the top differentially expressed genes between adenocarcinomas (ACs) and SCCs is P63. It is a well-known marker of squamous differentiation, but surprisingly, its expression is not widely used for this purpose. Our goals...

Results:  52.9% men, 57.5% smokers, acinar predominant most prevalent  EGFR mutation in 46.3% (55.9% 19del including 30.6% E746_A750 del; 41.4% L858R)  Interobserver variability for E746_A750del-specific ab: =0.86 (if score ≥2 considered +), =0.95 (score 3+), =0.43 (all scores)  Interobserver variability for L858R-specific protein ab: =0.73 (if score ≥2 considered +), =0.97 (score 3+),...

Ezrin-radixin-moesin-binding phosphoprotein 50 (EBP50) is a postsynaptic density-95/disc-large/zonula occludens-1 (PDZ) homologous domain-containing protein that is involved in cell signaling. EBP50 regulates cell apoptosis, proliferation and invasion. In the present study, the prognostic impact factor of EBP50 expression was evaluated using a quantum dot (QD)-based assay and immunohistochemist...

BACKGROUND Mutations in the p53 tumor suppressor gene (also known as TP53) have been detected in a wide variety of human cancers. In breast cancer, the presence of p53 gene alterations has been associated with worse prognosis. PURPOSE We compared a complementary DNA (cDNA)-based sequencing method and an immunohistochemical (IHC) method for their abilities to detect p53 mutations in breast can...

BACKGROUND HER2 testing in gastric cancer differs from testing in breast cancer because of inherent differences in tumor biology; gastric cancer more frequently shows HER2 heterogeneity and incomplete membrane staining. The aim of the present study was to evaluate the frequency and accuracy of detection of HER2 expression by application of standard criteria in Japanese patients with gastric can...

Fluorescent in-situ hybridization (FISH) and immunohistochemistry (IHC) constitute a pair of complimentary techniques for detecting gene amplification and overexpression, respectively. The advantages of IHC include relatively cheap materials and high sample durability, while FISH is the more accurate and reproducible method. Evaluation of FISH and IHC images is still largely performed manually,...

The purpose of this study was to investigate the effects of mild systemic hypoxia on inner haircell (IHC) and outer haircell (OHC) function. A number of studies have explored the effects of anoxia/hypoxia and ischemia of the inner ear [e.g. 1,2,3,4,5] but have not addressed whether there is a dissociation between IHC and OHC function during such an insult. To monitor OHC activity we recorded ot...