Triton X-100 is widely but not universally used in NADPH-diaphorase histochemical staining. We investigated its effect on the staining and examined nitroblue diformazan (NBF) production under the influence of Triton X-100. Exposure of opossum esophagus, intestine, and colon tissues to Triton X-100 before staining enhanced staining of nerve cells and fibers and suppressed staining of non-neural ...

Bestrophin-1 (Best-1) is an integral membrane protein, defects in which cause Best vitelliform macular dystrophy. Best-1 is proposed to function as a Cl- channel and/or a regulator of Ca++ channels. A tetrameric (or pentameric) stoichiometry has been reported for recombinant best-1. Using a combination of gel exclusion chromatography and velocity sedimentation we examined the quaternary structu...

An x-ray beam with energy of 20.5 keV has been efficiently focused down to a spot size as small as 90 nm390 nm by a Kirkpatrick–Baez reflecting mirrors device. The first mirror, coated with a graded multilayer, plays both the role of vertical focusing device and monochromator, resulting in a very high flux s231011 photons/sd and medium monochromaticity sDE /E,10−2d. Evaluation of the error cont...

In this study, we examined aspects of the 4 x 100 m relay that are amenable to mathematical analysis. We looked at factors that affect the time required to complete the relay, focusing on the performance of elite male athletes. Factors over which the individual athletes, and the team coach, can exercise some control are: the starting positions of the runners on legs 2, 3 and 4, the positions at...

Quasi-elastic light scattering spectroscopy was used to measure the mutual diffusion coefficient, D,, of Triton X-100 micelles in aqueous solution and the translational diffusion coefficient, D,, of mesoscopic probes in the same solutions. We apply conventional hydrodynamic treatments of diffision under the assumption that Triton X-100 minimal micelles are adequately represented as hard spheres...

We have demonstrated that structures down to 150 nm can be visualized in X-ray projection images using a nano-focus X-ray source. Due to their unlimited depth of focus, they don a limit on the specimen size in the case of 3D tomographic imaging. Further simulation studies have shown that optimization of the detector response curve and switching from a reflective Xray target to a transmission ta...