U87MG is a commonly studied grade IV glioma cell line that has been analyzed in at least 1,700 publications over four decades. In order to comprehensively characterize the genome of this cell line and to serve as a model of broad cancer genome sequencing, we have generated greater than 30x genomic sequence coverage using a novel 50-base mate paired strategy with a 1.4kb mean insert library. A t...

Abstract Rapeseed (Brassica napus L.) is a typical polyploid crop and one of the most important oilseed crops worldwide. With rapid progress on high-throughput sequencing technologies reduction in cost, large-scale genomic data specific have become available. However, raw sequence are mostly deposited read archive National Center Biotechnology Information European Nucleotide Archive, which free...

The present investigation was carried out to estimate indica-tropical japonica genome proportion in two newly developed wide compatible restorer lines (RP6367 and RP6368) derived through crosses study the molecular diversity among 12 rice genotypes namely, five promising indica restorers (RPHR1005, RPHR1096, IBL57, DR714-1-2 Akshayadhan), maintainers (IR58025B APMS6B), identified (RP6367and RP6...

Nuclear introns are commonly used as phylogenetic markers, but a number of issues related to alignment strategies, indel treatments, and the incorporation of length-variant heterozygotes (LVHs) are not routinely addressed when generating phylogenetic hypotheses. Topological congruence in relation to an extensive mitochondrial DNA multigene phylogeny (derived from 2,423 bp of 12S, 16S, ND4, and ...

Ginger (Zingiber officinale Rosc.) is an important herb of the family Zingiberaceae. It is accepted as a universal cure for a multitude of diseases in Indian systems of medicine and its rhizomes are equally popular as a spice ingredient throughout Asia. SNPs, the definitive genetic markers, representing the finest resolution of a DNA sequence, are abundantly found in populations having a lower ...

Background Insertions and deletions (indels) account for more nucleotide differences between two related DNA sequences than substitutions do, and thus it is imperative to develop a stochastic evolutionary model that enables us to reliably calculate the probability of the sequence evolution through indel processes. In a separate paper (Ezawa, Graur and Landan 2015a), we established the theoretic...

ALK inhibitor crizotinib has shown potent antitumor activity in children with refractory Anaplastic Large Cell Lymphoma (ALCL) and the opportunity to include ALK inhibitors in first-line therapies is oncoming. However, recent studies suggest that crizotinib-resistance mutations may emerge in ALCL patients. In the present study, we analyzed ALK kinase domain mutational status of 36 paediatric AL...