نتایج جستجو برای: مارکر rapd

تعداد نتایج: 6409  

Journal: :Genetics 1994
R V Kesseli I Paran R W Michelmore

A detailed genetic map has been constructed from the F2 population of a single intraspecific cross of Lactuca sativa (n = 9). It comprises 319 loci, including 152 restriction fragment length polymorphism (RFLP), 130 random amplified polymorphic DNA (RAPD), 7 isozyme, 19 disease resistance, and 11 morphological markers. Thirteen major, four minor linkage groups and several unlinked markers are i...

2016
M. Darwish

Three shrimps population (Penaeus semesulacatus, Penaeus japonicus,Meta Penaeus monoceros) were investigated for determination of the genetic structure by using (PCR-RAPD) and SDS-PAGE. The results obtained from RAPD technique showed the same percentages of similarity (100%) within the three studied population. On the other hand, the degrees of similarity detected by SDSPAGE were 90%, 87% and 8...

Journal: :Polish journal of microbiology 2007
Yasser Bakri Mohammed Imad Eddin Arabi Mohammed Jawhar

Random amplified polymorphic DNA (RAPD) analysis was used to evaluate genetic diversity among 13 soil Penicillium strains originating from widely dispersed areas. Twenty one of the 34 synthetic random primers were found to identify polymorphism in amplification products. The results show a high level of diversity of RAPD markers among the strains. All the strains could be identified by their ch...

Journal: :Revista iberoamericana de micologia 2007
Laura Baires-Varguez Alejandro Cruz-García Lourdes Villa-Tanaka Sergio Sánchez-García Luis Alberto Gaitán-Cepeda Luis Octavio Sánchez-Vargas Guillermo Quindós César Hernández-Rodríguez

The objective of this work was to compare the usefulness of a randomly amplified polymorphic DNA (RAPD) assay to that of the ATB ID32C kit (bioMérieux, France) for identification of different species of Candida isolated from clinical specimens. The RAPD-PCR patterns obtained with OPE-18 primer for identification of clinical isolates were consistent, and the different independent assays revealed...

Journal: :Epidemiology and infection 1995
P T Odinot J F Meis P J Van den Hurk J A Hoogkamp-Korstanje W J Melchers

PCR-based DNA fingerprinting was used to characterize 48 clinical isolates of Yersinia enterocolitica. The samples were examined by random amplified polymorphic DNA (RAPD-PCR) and inter-repeat PCR (IR-PCR). IR-PCR with two enterobacterial repetitive intergenic consensus primers resulted in patterns which were poorly discriminated; 2 of 11 arbitrary primers (RAPD-PCR) provided sufficient discrim...

Journal: :Microbiology research 2022

Wine quality is determined by the particular yeast strains prevailing at various stages of fermentation. Therefore, ability to make an easy, fast, and unambiguous discrimination yeasts strain level great importance. Here, tandem repeat-tRNA (TRtRNA) method with 5GAC or ISSR-MB primer sets random amplified polymorphic DNA (RAPD) analysis (GTG)3, R5, RF2 oligonucleotides were tested on non-Saccha...

Journal: :Acta Botanica Hungarica 2021

Genetic relationships among 24 genotypes of Hyophila involuta collected from five different natural populations Mount Abu (Rajasthan) is analysed using RAPD and SSR markers. Based on efficiency parameters calculated for each marker system such as polymorphic information content (RAPD = 0.34; 0.66), index 2.78; 2.62) resolving power 8.13; 2.23), the shows higher values some indices but microsate...

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه شهید چمران اهواز - دانشکده دامپزشکی 1389

مارکرهای dna از جمله مواد رایج در بیولوژی مولکولی می باشند.استانداردهای وزنی مولکولی dna ترکیبی از قطعات dna (دو رشته ای یا تک رشته ای) با اندازه شناخته شده می باشند. به صورت معمول، نمونه dna و یک مارکر در حفرات مجاور هم در ژل آگارز بارگذاری می شوند. dnaبه وسیله الکتروفورز در ژل جداشده و اندازه قطعات نمونه به وسیله مقایسه میزان حرکت با باندهای شناخته شده مارکر، معین می گردد. هدف از مطالعه حاضر ...

2005
RICHARD K. GROSBERG DONALD R. LEVITAN

CONTENTS SYNOPSIS INTRODUCTION WHAT ARE RAPDs Introduction Scoring RAPD Polymorphisms Sources of Polymorphism and Amplification Artifacts Population Genetic, Genealogical, and Phylogenetic Implications of Dominance and Cryptic Variation: A Preliminary Overview PROBLEMS WITH RAPD-PCR AND SOME REMEDIES Repeatability of Banding Patterns Within and Among Individuals Background Some Remedies Comigra...

Journal: :Applied and environmental microbiology 2010
Anne Holch Caroline Trebbien Gottlieb Marianne Halberg Larsen Hanne Ingmer Lone Gram

We determined mammalian cell invasion and virulence gene (inlA, inlB, and actA) sequences of Listeria monocytogenes strains belonging to a molecular subtype (RAPD 9) that often persists in Danish fish-processing plants. These strains invaded human placental trophoblasts less efficiently than other L. monocytogenes strains, including clinical strains, and they carry a premature stop codon in inl...

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