Mice lacking Niemann-Pick C1-Like 1 (NPC1L1) (NPC1L1(-/-)mice) exhibit a defect in intestinal absorption of cholesterol and phytosterols. However, wild-type (WT) mice do not efficiently absorb and accumulate phytosterols either. Cell-based studies show that NPC1L1 is a much weaker transporter for phytosterols than cholesterol. In this study, we examined the role of NPC1L1 in phytosterol and cho...

Abstract Background The expansion and metastasis of colorectal cancers are closely associated with the dynamic growth cancer stem cells (CSCs). This study aimed to explore possible effect LXR (a regulator glycolysis lipid hemostasis) in tumorgenicity human CD133 cells. Methods Human HT-29 + were enriched by MACS incubated agonist (T0901317) antagonist (SR9243) for 72 h. Cell survival was evalua...

ATP-binding cassette transporters ABCG5 (G5) and ABCG8 (G8) form a heterodimer that transports cholesterol and plant sterols from hepatocytes into bile. Mutations that inactivate G5 or G8 cause hypercholesterolemia and premature atherosclerosis. We showed previously that the two nucleotide-binding domains (NBDs) in the heterodimer are not functionally equivalent; sterol transport is abolished b...