Title of Dissertation: ASSEMBLY OF QUORUM SENSING PATHWAY ENZYMES ONTO PATTERNED MICROFABRICATED DEVICES Angela T. Lewandowski, Doctor of Philosophy, 2007 Directed By: Professor William E. Bentley, Department of Chemical and Biomolecular Engineering I report patterned protein assembly onto microfabricated devices using our unique assembly approach. This approach is based on electrodeposition of...

Autoinducer molecules are utilized by gram-negative and gram-positive bacteria to regulate density-dependent gene expression by a mechanism known as quorum sensing. PCR and DNA sequencing results showed that Campylobacter jejuni and Campylobacter coli possessed luxS, which is responsible for autoinducer-2 (AI-2) production. Using a Vibrio harveyi luminescence assay, the production of AI-2 was o...

Bacterial cell-to-cell communication (‘quorum sensing’) is mediated by structurally diverse, small diffusible signal molecules which regulate gene expression as a function of cell population density. Many different Gramnegative animal, plant and fish pathogens employ Nacylhomoserine lactones (AHLs) as quorum sensing signal molecules which control diverse physiological processes including biolum...

Quorum sensing (QS), or cell-to-cell communication in bacteria, is achieved through the production and subsequent response to the accumulation of extracellular signal molecules called autoinductors. The main role of QS is regulation of production of virulence factors in bacteria. Bacterial pathogenicity is often manifested by the expression of various cell-associated and secreted virulence fact...