This study was undertaken to investigate possible interrelationships between Ca2+ homeostasis and pH regulation in trout hepatocytes. Exposure of cells to Ca2+ mobilizing agents ionomycin (0.5 micromol l(-1)) and thapsigargin (0.1 micromol l(-1)) induced an increase in intracellular pH (pHi) that was dependent on Ca2+ influx from the extracellular medium as well as Ca2+ release from intracellul...

Short-chain fatty acids (SCFAs) are the major anions in the colonic lumen. Experiments studied how intracellular pH (pHi) of isolated colonocytes was affected by exposure to SCFAs normally found in the colon. Isolated crypt fragments were loaded with SNARF-1 (a fluorescent dye with pH-sensitive excitation and emission spectra) and studied in a digital imaging microscope. Intracellular pH was me...

The prediction made by L. O. Chua 45+ years ago (see: IEEE Trans. Circuit Theory (1971) 18:507-519 and also: Proc. IEEE (2012) 100:19201927) about the existence of a passive circuit element (called memristor) that links the charge and flux variables has been confirmed by the HP lab group in its report (see: Nature (2008) 453:80-83) on a successful construction of such an element. This sparked a...

The role of plasma membrane V-ATPase activity in the regulation of cytosolic pH (pHi) was determined for resident alveolar and peritoneal macrophages (m theta) from sheep. Cytosolic pH was measured using 2',7'-biscarboxyethyl-5,6-carboxyfluorescein (BCECF). The baseline pHi of both cell types was sensitive to the specific V-ATPase inhibitor bafilomycin A1. Bafilomycin A1 caused a significant (a...

OBJECTIVES Parental socioeconomic status (SES) exerts a substantial influence on children's health. The purpose of this study was to examine factors determining children's private health insurance (PHI) enrolment and children's healthcare utilization according to PHI coverage. METHODS Korea Health Panel data from 2011 (n=3085) was used to explore the factors determining PHI enrolment in child...

Objective Traumatic brain injury (TBI) occurs commonly in children. Repeat computed tomography (CT) follow up of TBI patients is often scheduled to identify progressive hemorrhagic injury (PHI). However, the utility of repeated CT scans, especially in children with mild TBI [Glasgow Coma Scale (GCS) scores of 13-15], has been debated. The purposes of the present study were to identify clinical ...

Hypoperfusion to the gut during cardiac arrest is an important clinical problem. The inability to control pH during metabolic stress, e.g. ischemia, leads to the disruption or halting of processes vital to balancing cellular metabolism. Alterations in cellular pH have been linked to changes in intramucosal permeability, which may result in the leakage of inflammatory mediators or bacteria, or b...

Dilepton spectra from the decay of phi mesons produced in heavy-ion collisions at SIS/GSI energies (∼ 2 GeV/nucleon) are studied in the relativistic transport model. We include phi mesons produced from baryon-baryon, pion-baryon, and kaon-antikaon collisions. The cross sections for the first two processes are obtained from an one-boson-exchange model, while that for the last process is taken to...

We create collectively jammed (CJ) packings of 50-50 bidisperse mixtures of smooth disks in two dimensions (2D) using an algorithm in which we successively compress or expand soft particles and minimize the total energy at each step until the particles are just at contact. We focus on small systems in 2D and thus are able to find nearly all of the collectively jammed states at each system size....

The present study demonstrates a unique mechanism for tumor cell-induced immunosuppression. In the presence of a nonsuppressive dose of tumor cells, generation of cytotoxic T cells in the mixed lymphocyte culture (MLC) is completely suppressed by adding exogenous (peritoneal) macrophages (PM phi) after the initiation of the MLC. This indicates that tumor cells can switch on a suppressor mechani...