omicron-Tyrosine (omicron-Tyr) was used as a specific biomarker for .OH radicals generated in biosystems. Specificity of omicron-Tyr as an .OH biomarker was based on previous studies in systems exposed to ionizing radiations. Fresh muscle tissue incubated with benzene for 1 hr at 38 degrees C exhibits formation of omicron-Tyr as seen in the cases of ethanol- and carbon tetrachloride-exposed sys...

Photosynthetic reaction centers from Blastochloris viridis possess Tyr-L162 located mid-way between the special pair chlorophyll (P) and the heme (heme3). While mutation of the tyrosine does not affect the kinetics of electron transfer from heme3 to P, recent time-resolved Laue diffraction studies reported displacement of Tyr-L162 in response to the formation of the photo-oxidized P(+•), implyi...

BACKGROUND In phenylketonuria (PKU), elevated blood phenylalanine (Phe) concentrations are considered to impair transport of large neutral amino acids (LNAAs) from blood to brain. This impairment is believed to underlie cognitive deficits in PKU via different mechanisms, including reduced cerebral protein synthesis. In this study, we investigated the hypothesis that impaired LNAA influx relates...

In rats, dietary supplementation with the amino acid tyrosine (TYR) prevents depletion of central catecholamines observed during acute environmental stress. Concomitant changes in the animals' behavioral responses to stress suggest that TYR might have similar effects on central catecholamines and cognition in humans exposed to environmental stress. This study aimed to determine if severe cold e...

We have investigated the sites of microtubule (MT) assembly in neurons during axon growth by taking advantage of the relationship between the proportion of tyrosinated alpha-tubulin (tyr-tubulin) in MTs and their age. Specifically, young (newly assembled) MTs contain more tyr-tubulin than older (more long-lived) MTs. To quantify the relative proportion of tyr-tubulin in MTs, cultured rat sympat...

Understanding the effects of processing pulses is required for their effective incorporation into livestock feed. To determine impact processing, Canadian peas, lentils, chickpeas, and faba beans, plus soybean meal (SBM; as a comparison), were ground fine coarse products pelleted at three different temperatures (60–65, 70–75, 80–85 °C). Grinding increased crude protein content in all ( P < 0...

The Tyr-MIF-1 family of neuropeptides (Tyr-MIF-1’s) includes MIF-1, Tyr-MIF-1, Tyr-W-MIF-1 and Tyr-K-MIF-1. Nowadays it is certain that they can modulate pain and some forms of stress-induced analgesia (SIA) by antiopiate and μ-specific processes. The objectives of the present study were twofold: 1) to determine the involvement of opioid system in analgesia induced by immobilization, cold and h...

The tyrosine fluorescence of the RecA protein is quenched by about 15% upon binding of the cofactor analog adenosine 5'-O-(3-thiotriphosphate) (ATP gamma S). This quenching is not observed with a modified RecA in which the tyrosine residue at position 264 (Tyr-264) is replaced for alanine by site-directed mutagenesis, a modification which also results in a decrease of binding affinity of cofact...

5' AAGCTTCCAC TACCTTGCCA CCCGCAATAA GAACGATTAC TTCTCCCTCG CCTT 54 Kec Pro tie Thr Asn 141 CTACCA CCTAAAGATC TCCCTCTTAT TTTTAGOTTG AACTCGTATA AACCAAAATT AATTACACCA GATAAA ATG CCA ATT ACA AAT -35 -10 BBS fut Lys ThT Hec Leu Il« Thr Tyr Ala Aap Ser Leu Gly Lye Asa Leu Lys Glu Leu Asn Glu A«n H e Glu Agn Tyr AAA ACA ATG TTC ATT ACT TAC GCA CAC ACT TTC OGT AAA AAT TTG AAA CAA TTG AAT GAA AAT ATT GAG...