By a suppression subtractive hybridization based method, nine novel Mycobacterium avium subsp. paratuberculosis (M. paratuberculosis) fragments of between 318 and 596 bp have been identified and characterized. Database search revealed little or no similarity with other mycobacteria. The uniqueness and diagnostic potential of seven of these fragments in relation to M. paratuberculosis closest re...

Pavlas, M.: Heat Resistance and Vinllence ofSerovars of the Mycobacterium avium Complex. Acta vet. Bmo 1997,66: 249-252. Heat resistance and virulence tested in chickens were investigated in 151 strains classified with the Mycobacterium avium complex. The highest differences in heat resistance between virulent and avirulent strains were found at 2-h exposure to 60°C. Under such conditions, 92% ...

Fluorescent acid-fast microscopy (FAM) was used to enumerate intracellular Mycobacterium avium, Mycobacterium intracellulare, and Mycobacterium scrofulaceum in the ciliated phagocytic protozoan Tetrahymena pyriformis. There was a linear relationship between FAM and colony counts of M. avium cells both from cultures and within protozoa. The Ziehl-Neelsen acid-fast stain could not be used to enum...

The outer membrane proteins of Bordetella avium were examined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Sarkosyl-insoluble outer membrane protein-enriched profiles from 50 virulent B. avium isolates, containing major 21,000- and 37,000-molecular-weight proteins (21K and 37K proteins, respectively) and at least 13 less intensely stained proteins with molecular weights ranging...

Mycobacterium avium is an environmental organism and opportunistic pathogen with inherent resistance to drugs, environmental stresses, and the host immune response. To adapt to these disparate conditions, M. avium must control its transcriptional response to environmental cues. M. avium forms biofilms in various environmental settings, including drinking water pipes and potable water reservoirs...

A PCR method previously developed for typing Mycobacterium avium was used to characterize the genetic diversity of M. avium strains isolated from swine (n = 90) and humans (n = 24). The strains were identified with IS901 PCR and IS1245 PCR: 38 strains were of IS901+ and IS1245+ genotype (M. avium subsp. avium) and 76 strains were of IS901– and IS1245+ genotype (M. avium subsp. hominissuis). All...

The effects of preharvest calcium chloride (CaCl2) treatment on some quality characteristics and bioactive compounds sweet cherry fruit (Prunus avium L. cvs. 0900 Ziraat, Sweetheart Merton Late) were investigated. CaCl2 (1%) solution was sprayed to the trees at 21 35 days after full bloom stage. served as control treated with distilled water same days. remarkably suppressed respiration rate inc...

Mycobacterium avium complex (MAC) species cannot be discriminated by the usual methods of biochemical identification of mycobacteria. This study showed that amplification by PCR of DT1 and DT6, two single copy sequences identified in the genome of M. avium serotype 2, the insertion sequence IS1245, found to be consistently present in M. avium strains and the heat-shock protein gene hsp65, follo...

HPLC, which is gaining its place as identification tool in mycobacteriology laboratories, has been proposed to distinguish Mycobacterium paratuberculosis from Mycobacterium avium. We had reported no significant difference between M. avium and M. paratuberculosis reference strain ATCC 19698. Because of the advantages offered by such a method, we enlarged our observations to include more isolates...

BACKGROUND Mycobacterium avium subspecies paratuberculosis causes systemic infection and chronic intestinal inflammation in many species including primates. Humans are exposed through milk and from sources of environmental contamination. Hitherto, the only vaccines available against Mycobacterium avium subspecies paratuberculosis have been limited to veterinary use and comprised attenuated or k...