A stroma-dependent cell line (HB-1) was established from myelogenous leukemic cells of CBA/N mouse. Characterization of the cells showed that HB-1 proliferated on hematopoietic supportive stromal cells (MS-10), but did not survive or proliferate on hematopoietic nonsupportive cells (MS-K). Direct contact between HB-1 and MS-10 appears to be necessary for HB-1 to proliferate on MS-10. We found t...

A new cell line named the tilapia heart (TAH-11) was established from of (Oreochromis niloticus) by enzymatic digestion and mechanical separation. The TAH-11 has been stably subcultured for over 80 generations resuscitated after being frozen in liquid nitrogen six months, with exuberant growth. optimal culture condition is L-15 medium supplemented 10% fetal bovine serum at 25 °C. Sequencing mit...

objective(s): the aim of this study was to investigate the methylation status and mrna expression levels of p15, death-associated protein kinase (dapk), and suppressor of cytokine signaling-1 (socs1) genes in multiple myeloma (mm). materials and methods: the bone marrow samples of 54 mm patients were collected and the methylation status of the p15, dapk, and socs1 gene promoter regions was dete...

conclusions some results of the correlation coefficients are not the same with visualization. the reason may be due to the small number of data. materials and methods in the foundation-application study, we constructed two-way gene networks using nonparametric methods, such as spearman’s rank correlation coefficient and blomqvist’s measure, and compared them with pearson’s correlation coefficie...

Introduction: Endometriosis is a prevalent gynecological disorder among women which is diagnosed by the growth of endometrial tissue outside of uterus and is mainly accompanied by severe pelvic pain and infertility. P53 also known as cellular tumor antigen P53 inside codons 11, 72 and 248 are contained with single nucleotide changes in which tends to be nearly rampant.This will probably b...