Environmental biosurveillance and microbial ecology studies use PCR-based assays to detect and quantify microbial taxa and gene sequences within a complex background of microorganisms. However, the fragmentary nature and growing quantity of DNA-sequence data make group-specific assay design challenging. We solved this problem by developing a software platform that enables PCR-assay design at an...

introduction: helicobacter pylori (h. pylori), is a bacterium responsible for upper gastrointestinal tract diseases. the 16s rrna is a common h. pylori gene which are usually preferred for diagnosis purpose. the aim of this study was to determine the prevalence and abundance of 16s rrna in fecal samples and also evaluate correlation between the level of 16s rrna and activities of the cytokines,...

BACKGROUND AND OBJECTIVE Conventional identification of mycobacteria is achieved by standard biochemical tests that are time consuming, laborious and is not always conclusive. This study was thus undertaken to standardize a simple, rapid and cost-effective polymerase chain reaction based restriction fragment length polymorphism (PCR-RFLP) using primers coding for the 16S - 23S rRNA spacer regio...

BACKGROUND To determine the presence of Helicobacter species in the liver biopsy specimens from children with various chronic liver diseases as data in adult literature suggests a possible role of these bacteria in their pathogenesis. MATERIALS AND METHODS Paraffin sections of 61 liver biopsies of pediatric patients with miscellaneous diseases and autopsy liver tissue from 10 control subjects...

background: acute bacterial meningitis is among serious infections of the central nervous system (cns). the early diagnosis and empiric antibiotic treatments have led to a reduction in morbidity and mortality rates. pcr and the enzymatic digestion of 16s rdna fragment following the pcr by universal primers led up fast and sensitive determination. the aims of the present study was to improve our...

Studies were conducted on the improvement of A. culicicola identification. This species is phenotypically very similar to A. veronii biotype sobria, A. sobria, and A. allosaccharophila. The sequences of 16S rDNA of A. culicicola isolates show the highest similarity with A. jandaei, A. veronii, and A. caviae. Digestion of 16S rDNA PCR product with AluI and MboI restriction endonucleases allowed ...

conclusions: this study detected multiple drug resistance in pseudomonas aeruginosa including resistance to β-lactams, aminoglycosides, and fluoroquinolones. therefore, identification of drug resistance patterns in p. aeruginosa and detection of pan-resistant producing isolates are of great importance in prevention and control of infections in burn center ward. background: serious infections by...

introduction and aims: until today human stomach is the only recognized habitat of h. pylori . however, recruitment of dna-based methods has made possible the detection of h. pylori in water and oral cavity, thus suggesting faecal-oral and oral-oral routes for transmission of h. pylori , respectively. in this study yeast has been proposed as a common vector for transmission of h. pylori . thus ...