In a search for methods for subtyping of Bartonella henselae in clinical samples, we amplified and sequenced a 701-bp region in the 3' end of the ftsZ gene in 15 B. henselae isolates derived from cats and humans in the United States and Europe. The ftsZ sequence variants that were discovered were designated variants Bh ftsZ 1, 2, and 3 and were compared with 16S rRNA genotypes I and II of the s...

Philosamia ricini, the Indian eri silkworm, contributes significantly to the production of commercial silk and is widely distributed in the Brahmaputra valley of the North-Eastern India. Strains of P. ricini show wide variation in their phenotypic traits and are commercially exploited in these places because of their high silk yield potential. In this study, we have analyzed the partial mitocho...

Actinomycetes are inexhaustible producers of commercially valuable metabolites, are continually screened for beneficial compounds. The taxonomic and phylogenetic study of novel actinomycetes strains are mostly based on conventional methods and primary DNA structure of 16s rRNA. Although 16s rRNA sequence is well accepted in phylogeny studies, its secondary structures have not been widely used. ...

During 2021 surveys, greengage witches’-broom (GWB) and decline (GD) diseases were respectively observed in Fars Yazd provinces of Iran. Presence phytoplasma the symptomatic plants was confirmed by PCR amplifications 16S rRNA gene. Sequence analyses showed affiliation GWB GD phytoplasmas to‘Candidatus Phytoplasma phoenicium’ (16SrIX-B) and‘Ca. P. aurantifolia’ (16SrII-C) related strains, respec...

BACKGROUND Nosocomial infection is one of the most common complications within health care facilities. Certain studies have reported outbreaks resulting from contaminated hospital environments. Although the identification of bacteria in the environment can readily be achieved using culturing methods, these methods detect live bacteria. Sequencing of the 16S ribosomal RNA (16S rRNA) gene is reco...

The nucleotide sequence of Escherichia coli DNA at both ends of the gene for 16S rRNA has been determined for two rRNA operons, rrnD and rrnX. The 400 nucleotides we have examined exhibit only one base change between rrnD and rrnX. Within the 160 nucleotides that precede mature 16S rRNA sequences are cleavage sites for several E. coli endonucleases, including RNase III. A 240-nucleotide segment...

High-throughput culturing (HTC) methods that rely on dilution to extinction in very-low-nutrient media were used to obtain bacterial isolates from Crater Lake, Oregon. 16S rRNA sequence determination and phylogenetic reconstruction were used to determine the potential ecological significance of isolated bacteria, both in Crater Lake and globally. Fifty-five Crater Lake isolates yielded 16 diffe...