Background The pathogenesis of Spondyloarthritis (SpA) remains unknown but its strong association with some alleles HLA-B*27 is peculiar. arthritogenic antigen hypothesis assumes the existence specific peptides presented by risk-conferring to antigen-specific CD8+ T cells, which then initiate or sustain autoimmune reactions. Several studies analyzing cell receptor (TCR) repertoire found preferr...

The murine T-cell surface molecules Lyt-2 and L3T4 play a role in the activation of antigen-specific T cells. The currently accepted model for the function of these molecules proposes that Lyt-2 and L3T4 increase the overall avidity of the interaction between the T-cell antigen receptor and antigen in association with the major histocompatibility complex (MHC) molecules on the antigen-presentin...

The IL-2/IL-2Ralpha (CD25) axis is of central importance for the interplay of effector and regulatory T cells. Nevertheless, the question how different antigen loads are translated into appropriate IL-2 production to ensure adequate responses against pathogens remains largely unexplored. Here we find that at single cell level, IL-2 is binary (digital) and CD25 is graded expressed whereas at pop...

Previous studies from our laboratory established that C-ASWS, an alkali-soluble, water-soluble extract from cell walls of Coccidioides immitis, protects mice against lethal challenge with this fungus. The C-ASWS extract contains a glycosylated protein, designated antigen 2 (Ag2), and a polysaccharide antigen. We recently cloned Ag2 cDNA and showed that the recombinant fusion protein elicited st...

Objective: As an alternative to RT-qPCR assays used in the diagnosis SARS-CoV-2, antigen-detecting rapid diagnostic tests (Ag-RDTs) are available for qualitative detection of SARS-CoV-2 nasopharyngeal swab samples. The aim this study was assess accuracy and reliability Ag-RDTs as a method detecting positive cases within given population.
 Methods: In first phase investigation, 357 samples ...

Background: Cystic echinococcosis (CE), as a chronic parasitic disease, is a major health problem in many countries. The performance of the currently available serodiagnostic tests for the diagnosis of CE is unsatisfactory. Objective: The current study aimed at sub-cloning a gene, encoding the B8/1 subunit of antigen B (AgB) from Echinococcus granulosus, using gene optimization for the immunodi...

Administration in vivo of recombinant interleukin 2 (rIL-2) to mice induces a polyclonal IgM response. When co-administered with a specific antigen, rIL-2 can enhance concentrations of murine IgM antibodies specific for the antigen by fivefold within 7 d of initial treatment. IgM antibodies that are induced after injection of rIL-2 include antibodies specific for J5, a cell wall core lipopolysa...

The aim of the present study was to provide a national estimate for transmission COVID-19 Cases in public and private schools Benghazi city Eastern region Libya. A multistage procedure followed obtain representative sample students teaching staffs at randomly selected across resultant consisted 101 schools, 808 students, 202 staff members. Data were collected on age, sex, class, symptoms COVID1...

Abstract In this study, whole blood samples and ticks were collected from 57 capybaras in recreational areas the Federal District, Brazil, aiming to investigate presence of Rickettsia spp. using polymerase chain reaction (PCR) indirect immunofluorescence (IFAT) assays. None capybara yielded rickettsial DNA by PCR. Among 55 serum tested IFAT, 53 (96.3%) reacted these, 21 (39.6%) identified R. be...