The suppressive effect of (–)-epigallocatechin gallate (EGCG), the major polyphenolic constituent present in green tea, on aflatoxin B1 (AFB1)-induced chromosome aberrations (CA) in rat bone marrow cells was studied. The administration of EGCG 24 hr before the AFB1 injection significantly suppressed AFB1-induced CA. The suppression was observed 18 hr, 24 hr and 48 hr after the AFB1 treatment bu...

Aflatoxin B1 (AFB1) is the most toxic and common among major types of aflatoxins. AFB1 hepatoxic has been implicated in an increase risk hepatocellular carcinoma, also effect AFs on brain chemistry have documented. Probiotics health benefits provide powerful for body brain. The present study was designed to reveal efficiency Bacillus subtilis NS4182-01 against hematological, biochemical histopa...

Two commercial materials, a bamboo charcoal (BC) and a smectite clay (SC), were assessed in vitro with aflatoxin B1 (AFB1) in an equilibrium adsorption test. The adsorption capacity and proportion adsorbed (0.381 μg/mg, 0.955) for BC were greater than for SC (0.372 μg/mg, 0.931). The effects of in vitro ruminal fermentation of hay-rich feed incubated with 1.0 μg/mL AFB1 for 0-10 g/L doses of BC...

Aflatoxin B1 (AFB1) and sterigmatocystin (ST) are two hepatocarcinogenic mycotoxins that are commonly coexisted in cereal grains, and their co-proapoptotic activity in HepG2 cells was studied. The values of IC50, which is the dosage of mycotoxin resulting in a 50% cell growth inhibition measured by a sulforhodamine B (SRB) colorimetric assay, were 16.9 μM and 7.3 μM for AFB1 and ST, respectivel...

Aflatoxin contamination of foods and livestock feeds is an ongoing problem. In this research, NovaSil PLUS (NSP), a calcium montmorillonite clay that is commonly used as an anticaking agent in feeds, was evaluated for its ability to bind aflatoxin B1 (AfB1) in vitro and to prevent the onset of aflatoxicosis and vitamin A depletion in broiler chicks in vivo. Isothermal analyses were conducted wi...

Aflatoxins are natural toxic compounds produced by fungi such as Aspergillus flavus and Aspergillus parasiticus. Due to higher temperature and humidity, fungi are continuously grown and contaminated the cereals and cereal by-products with aflatoxin B1 (AFB1). Dairy cows fed AFB1 contaminated feeds can produce milk contaminated with aflatoxin M1 (AFM1). Therefore, the aim of this study was to in...

background: this study aims to evaluate the efficacy of silybum marianum seeds (smss) on blood biochemical profile of broiler chickens contaminated with aflatoxin b1 (afb1).  methods: combinations of three levels of afb1 (0, 250 and 500 ppb) with three levels of smss (0, 0.5, and 1.0 %) were incorporated into the basal diet (corn and soybean meal). the effect of nine experimental treatments was...

Through the present study, we produced a monoclonal antibody against aflatoxin B1 (AFB1) using AFB1- carboxymethoxylamine BSA conjugates. One clone showing high binding ability was selected and it was applied to develop a direct competitive ELISA system. The epitope densities of AFB1-CMO against BSA and KLH were about 1 : 6 and 1 : 545, respectively. The monoclonal antibody (mAb) from cloned hy...

Aflatoxin B1 (AFB1) is a food contaminant with detrimental impact on human and animal health. Intervention approaches focus on preand post-harvest measures to reduce AFB1 levels in crops or on the individual level to modulate bioactivation and excretion of AFB1 or reduce its bioavailability. Probiotic bacteria have been identified as a potential means to reduce availability of AFB1 as well as o...