NRH:Quinone oxidoreductase 2 (NQO2) is an enzyme that catalyzes the reductive metabolism of quinones. C57BL/6 NQO2-/- mice lacking NQO2 gene expression were generated in our laboratory. The dorsal skin of NQO2-deficient mice was exposed to 7,12-dimethylbenz(a)anthracene (DMBA) or benzo(a)pyrene alone (complete carcinogen) or with 12-O-tetradecanoylphorbol-13-acetate (TPA) (initiation/promotion ...

In the hormonally responsive 7,12-dimethylbenz(a)anthracene (DMBA)- or N-nitrosomethylurea (NMU)-induced mammary carcinomas, regulatory mechanisms have been altered such that these tumors retain their hormonal dependence for growth but possess only a limited ability to synthesize the mammary gland-specific milk proteins. Quantitation of casein mRNA levels revealed that very low levels of casein...

Aim of the present study was to investigate the protective effect of Clerodendron inerme on cellular integrity by measuring the status of glycoconjugates, lipids, osmotic fragility, and membrane bound enzyme activity in 7,12-dimethylbenz (a) anthracene (DMBA)-induced oral carcinogenesis. Oral squamous cell carcinoma was induced in the buccal pouch of Syrian golden hamsters by painting with 0.5%...

Aim of the present study was to investigate the anticlastogenic and antigenotoxic effect of andrographolide in the bone marrow cells of golden Syrian hamsters treated with 7,12dimethylbenz(a)anthracene (DMBA). The frequency of bone marrow micronucleated polychromatic erythrocytes (MnPCEs) was used as cytogenetic end point to assess the anticlastogenic effect of andrographolide in hamsters treat...

Clinical studies suggest that maternal smoking during pregnancy can reduce the crown size of the child's teeth. Delayed dental age compared with chronological age has also been reported in children whose parents smoke. Among the main components of tobacco smoke are nonhalogenated polycyclic aromatic hydrocarbons (PAHs), many of which are highly toxic. Humans are exposed to PAH compounds mainly ...

Female rats of ten different inbred strains were tested for their suscep tibility to 7, 12-dimethylbenz|a|anthracene (DMBA)-induced mammary adenocarcinogenesis. Five of these strains (OM. NSD, \VI. LEWIS, BUFF) were demonstrated to be highly susceptible to DMBA exposure developing >2 continuously growing, macroscopically detectable mam mary adenocarcinomas/rat following gastric intubation with ...

We compared the profile of ras gene mutations in spontaneous CD-1 mouse liver tumors with that found in liver tumors that were induced by a single i.p. injection of either 7,12-dimethylbenz(a)anthracene (DMBA), 4-aminoazobenzene, N-hydroxy-2-acetylaminofluorene, or N-nitrosodiethylamine. By direct sequencing of polymerase chain reaction-amplified tumor DNA, the carcinogen-induced tumors were fo...

Mouse ovarian aryl hydrocarbon hydroxylase (AHH, EC 1.14.14.2) activity was measured in control mice and in DBA/ 2N (hereafter called D2) and C57BL/6N (hereafter called B6) mice treated with 3-methylcholanthrene (MC). Basal ovarian AHH activity was similar in both strains (3 pmol/mg/min). Ovarian AHH was induced 2to 3-fold in B6 mice after MC treatment, while no change was observed in similarly...

Many mouse models of breast cancer have been developed that closely replicate human breast cancer subtypes at the genomic level. In order to develop vaccines that could prevent a broad spectrum of breast cancer subtypes, a combination of different spontaneous tumor models are needed. Transgenic models are available that mimic HER2+/luminal breast cancer (TgMMTV-Neu) and triple negative/basal-li...