2 cells

The aim of this study was to isolate mouse embryonic stem cells from late blastocyst stage embryos and to use them as a model system for the study of hematopoietic induction outside the embryo by coculturing of embryonic stem cells with bone marrow stromal cells. Blastocyst stage embryos from pregnant NMRI mice were obtained and cultured for 1-2 days in DMEM medium. The inner cell masses formed...

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Nanofiber Expansion of Umbilical Cord Blood Hematopoietic Stem Cells

Journal: Iranian Journal of Pediatric Hematology and Oncology 2015

A Allahverdi, F skandari, H Nasiri, H Zare-Zardini, M Azad, M Soleimani, N Kalantari,

Background The aim of this study was the ex vivo expansion of Umbilical Cord Blood hematopoietic stem cells on biocompatible nanofiber scaffolds. Materials and Methods CD133+ hematopoietic stem cells were separated from umbilical cord blood using MidiMacs (positive selection) system by means of monocolonal antibody CD133 (microbeads) subsequently, flowcytometry method was done to asses...

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gardenia jasminoides extracts and gallic acid inhibit lipopolysaccharide-induced inflammation by suppression of jnk2/1 signaling pathways in bv-2 cells

Journal: :iranian journal of basic medical sciences 0

wen-hung lin department of technology management, chung hua university, hsinchu, taiwan heng-hung kuo department of finance, chung hua university, hsinchu, taiwan li-hsing ho department of technology management, chung hua university, hsinchu, taiwan ming-lang tseng department of biotechnology, yuanpei university, hsinchu, taiwan an-ci siao office of physical education, chung hua university hsinchu, taiwan chang-tsen hung department of health and leisure management, yuanpei university, hsinchu, taiwan

objective(s): gardenia jasminoides ellis (gj, cape jasmine fruit, zhi zi) has been traditionally used for the treatment of infectious hepatitis, aphthous ulcer, and trauma; however, the direct evidence is lacking. materials and methods:we investigated the effect of the gj extract(gj) and gallic acid (ga) on lipopolysaccharide (lps) induced inflammation of bv-2 microglial cells and acute liver i...

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