the present study reports an efficient protocol for isolation and regeneration of protoplasts from callus of fritillaria imperialis l. there is no published method recommended for protoplast isolation and regeneration from fritillaria imperialis l. a range of factors, which influence the success of isolation and regeneration of f. imperialis protoplasts, were investigated. from the results obta...

background and objectives : there are limitations in time and technique for isolation of brucella from patients. we developed a new brucella culture medium and evaluated its efficiency compared to bactec blood culture system and serology. materials and methods: a bi-phasic medium containing urea agar and brain heart infusion was formulated. appearance of clear red color in liquid phase was the ...

This study uses a setup involving scanning electron microscopy (SEM) to examine the infiltration behaviour and mechanism of formation filter cake from sand-modified bentonite slurry. We began by determining whether can be formed using water–bentonite slurry in samples fine, medium, coarse sand (Series 1). Water–bentonite–sand slurries with different mass ratios fine (B:S) were subsequently test...

Two recent intratracheal instillation toxicology studies in rats clearly show that a naturally occurring quartz, with occluded crystal surfaces (quartz isolate), produced significantly less inflammatory response than a crushed reference quartz (DQ12). Respirable-size quartz isolate was isolated from bentonite parent rock, without crushing or the use of chemicals, to ensure that the surface prop...

Transmissible spongiform encephalopathies (TSEs, prion diseases) are a class of fatal neurodegenerative diseases affecting a variety of mammalian species including humans. A misfolded form of the prion protein (PrP(TSE)) is the major, if not sole, component of the infectious agent RecentTSE outbreaks in domesticated and wild animal populations have created the need for safe and effective dispos...

This study employed 16S rRNA gene amplicon pyrosequencing to examine the hypothesis that chemolithotrophic Fe(II)-oxidizing bacteria (FeOB) would preferentially colonize the Fe(II)-bearing mineral biotite compared to quartz sand when the minerals were incubated in situ within a subsurface redox transition zone (RTZ) at the Hanford 300 Area site in Richland, WA, USA. The work was motivated by th...

Flagellate infections were found in 1,063 of 18,895 sand flies collected in the states of Amazonas, Pará, Rondonia and Acre, Brazil. Infection rates were 13.4% (species group Shannoni); 7.5% (subgenus Nyssomyia); 6.7% (subgenus Lutzomyia series Cruciata); 0.5% (genus Psychodopygus) and 3.1% for other sand flies (various subgenera). Leishmania braziliensis guyanensis and L. mexicana amazonensis ...