Transfer of an aromatic chromophore from the interior of a protein into the water solvent upon denaturation of the protein produces an absorption change throughout the ultraviolet region, 215 to 320 rnp, approximately 6 times the magnitude of that produced by transfer of the same chromophore from 20% ethylene glycol into water. In the 230 rnE.1 wave length region, the transfer of an indole chro...

The NMR structures of the recombinant human prion protein, hPrP(23-230), and two C-terminal fragments, hPrP(90-230) and hPrP(121-230), include a globular domain extending from residues 125-228, for which a detailed structure was obtained, and an N-terminal flexibly disordered "tail." The globular domain contains three alpha-helices comprising the residues 144-154, 173-194, and 200-228 and a sho...

Objective of this study was to determine the effects of varying dietary cation-anion differences (DCAD) in prepartum period on milk fever, subclinical hypocalcemia and negative energy balance in dairy cows of Urmia, Iran. Ninety nine Holstein cows assigned to three groups (n=33) were fed diets with DCAD of -24 (anionic), +80 (cationic) and +230 (cationic) mEq/Kg dry matter for 3 weeks before ex...

BACKGROUND Postpartum sexual abstinence until when the child is weaned from breast milk is deeply rooted in most cultures in Nigeria. AIM The study aimed at describing the current sexual practices of postpartum women, sexual morbidity, contraceptive prevalence and predictive factors for early postpartum sexual intercourse and associated sexual problems in our setting. SUBJECTS AND METHODS T...

The NMR structures of the recombinant 217-residue polypeptide chain of the mature bovine prion protein, bPrP(23-230), and a C-terminal fragment, bPrP(121-230), include a globular domain extending from residue 125 to residue 227, a short flexible chain end of residues 228-230, and an N-terminal flexibly disordered "tail" comprising 108 residues for the intact protein and 4 residues for bPrP(121-...

The amyloid cascade hypothesis of Alzheimer's disease (AD) posits that the generation of β-amyloid (Aβ) triggers Tau neurofibrillary pathology. Recently a "17 kD" calpain-induced Tau fragment, comprising residues 45-230 (molecular weight [MW], 18.7 kD), was proposed to mediate Aβ-induced toxicity. Here, we demonstrate that the "17 kD" fragment is actually much smaller, containing residues 125-2...

PURPOSE To report a new technique to explore peripheral vitreous-retinal tractions in patients with acute posterior vitreous detachment. MATERIAL AND METHODS A population of 65 patients with acute posterior vitreous detachment was studied by panfunduscopic SLO 230 lens and HRA-II to detect peripheral retinal tears and vitreous-retinal tractions. RESULTS In our study, using indirect ophthalm...

The newly developed multireceptor somatostatin analogs pasireotide (SOM230), octreotide and somatoprim (DG3173) have primarily been characterized according to their binding profiles. However, their ability to activate individual somatostatin receptor subtypes (sst) has not been directly assessed so far. Here, we transplanted the carboxyl-terminal phosphorylation motif of the sst(2) receptor to ...