ANG II is capable of stimulating expression of immediate early genes such as egr-1 and c-fos in a variety of cultured cells, including cells of renal origin. To investigate whether ANG II can stimulate early growth response gene expression in vivo, we studied the effects of acute renal artery infusion of low-dose ANG II (2.5 ng small middle dot kg(-1) small middle dot min(-1)) or vehicle on the...

Ajulemic acid is a synthetic analog of Δ(8)-THC-11-oic acid, the terminal metabolite of Δ(8)-THC. Unlike Δ(9)-THC, the psychoactive principle of Cannabis, it shows potent anti-inflammatory action and has minimal CNS cannabimimetic activity. Its in vitro metabolism by hepatocytes from rats, dogs, cynomolgus monkeys and humans was studied and the results are reported here. Five metabolites, M1 to...

I could subtitle this feature “Kate’s Cool Video Clips” because the three Molecular Biology of the Cell (MBC) movies I review here were suggested by Kate Durda, a biology major at Colby College in Maine, who is compiling an index of MBC videos for ASCB. On a technical note, many of the video records I review in these features were obtained by “time-lapse photography”: that is, the images were c...

OBJECTIVE The potential insulin-sensitizing function of angiotensin II type 1 receptor blockade (ARB) with regard to selected adipokines is not fully explained so far. Our study aimed to explore the influence of acute hyperinsulinaemia and acutely induced ARB on resistin and adiponectin concentrations and expressions in healthy subjects. DESIGN AND METHODS Plasma adipokines were measured: 1) ...

We present the details of the first laparoscopic transplantation of a kidney from a living, related donor, performed April 16, 2009. Surgical and functional results were acceptable. Surgical time was 240 min (53 min for vascular suture), with blood loss of 300 cm(3) and a hospital stay of 14 d. Serum creatinine at discharge was 73 mmol/l. Laparoscopic kidney transplantation is a complex techniq...

Let Gn := ( f : f(t) = n X j=1 aje −(t−λj) , aj , λj ∈ R ) . In this paper we prove the following result. Theorem (Remez-Type Inequality for Gn). Let s ∈ (0,∞). There is an absolute constant c1 > 0 such that exp(c1(min{ns, ns2} + s)) ≤ sup f ‖f‖R ≤ exp(240(min{n1/2s, ns2} + s)) , where the supremum is taken for all f ∈ Gn satisfying m ({t ∈ R : |f(t)| ≥ 1}) ≤ s . We also prove the right higher ...

Twenty women, undergoing lower abdominal surgery, were allocated randomly to receive a continuous extradural infusion of either 0.125% bupivacaine or placebo at a rate of 15 ml h-1. All had received an intra-operative extradural block. Pain scores were recorded at 30, 60, 90, 120, 150, 180, 240 and 360 min after surgery. From 150 min onwards there was a significant benefit for those receiving t...

We demonstrated a rapid immunoassay for detection of cat cystatin C (cCys-C) which is an important marker for chronic kidney disease in cats, using immuno-pillar chips. The required amount of reagent solution is 200 times smaller than that for the conventional ELISA in the 96-well microplate (0.5 μL versus 100 μL). In addition, the total assay time in the proposed method is more than 12 times s...