We examined 26 atherosclerotic lesions and 14 nondiseased aorta specimens to detect the periodontopathogenic part of the bacterial 16S rRNA locus by PCR. Treponema denticola sequence of the 16S rRNA locus was found in 6 out of 26 DNA samples (23.1%) from the formalin-fixed, paraffin-embeded atherosclerotic lesions obtained during surgery but not in any of the 14 nondiseased aorta samples from d...

Ribosomal genes are strongly regulated dependent on growth phase in all organisms, but this regulation is poorly understood in Archaea. Moreover, very little is known about growth phase-dependent gene regulation in Archaea. SSV1-based lacS reporter gene constructs containing the Sulfolobus 16S/23S rRNA gene core promoter, the TF55α core promoter, or the native lacS promoter were tested in Sulfo...

The bacterial strain LH2-2(T) was isolated from freshwater of Longhu Lake, a slightly alkaline lake (pH 8.8) in north-east China. Cells of strain LH2-2(T) were Gram-staining-negative, non-spore-forming rods, 0.3-0.5 µm wide and 2.0-4.0 µm long. Cells were motile by means of a single polar flagellum. The strain was strictly aerobic and heterotrophic and oxidase- and catalase-positive. Growth occ...

concentrations of zn, cu, cr, cd, pb and fe in surface marine sediments of the southern coast of sfax were studied in order to understand current metal contamination at the outlet of a mixed industrial and domestic wastewater effluent discharged into the seawater since 1953. we found that heavy metal concentrations (mg/kg dry weight) in surface marine sediments varied from 42 to 391 for zn, 15 ...

concentrations of zn, cu, cr, cd, pb and fe in surface marine sediments of the southern coast of sfax were studied in order to understand current metal contamination at the outlet of a mixed industrial and domestic wastewater effluent discharged into the seawater since 1953. we found that heavy metal concentrations (mg/kg dry weight) in surface marine sediments varied from 42 to 391 for zn, 15 ...

Occurrence of cyst nematode Heterodera daverti Wouts and Sturhan 1978 (Wouts & 1978) is reported from Australia for the first time. Cysts were recovered soil samples collected a hop farm in Merriang, Victoria. Morphological morphometric characteristics cysts second stage juveniles match those described this species other parts world. Identification was further supported by molecular evidence th...

The biogenesis of ribosomes in vivo is an essential process for cellular functions. Transcription of ribosomal RNA (rRNA) genes is the rate-limiting step in ribosome biogenesis controlled by environmental conditions. Here, we investigated the role of folate antagonist on changes of DNA double-strand breaks (DSBs) landscape in mouse embryonic stem cells. A significant DSB enhancement was detecte...

background and objectives: the development of molecular biological techniques provide a method for direct detection and identification of pathogens. this study attempted to compare bacterial culture and pcr to detect s. agalactiae in cattle milk samples. the pcr method was based on using primers v1 and v2 derived from the 16s rrna gene. materials and methods: one hundred milk samples were colle...

Teniposide [4'-demethylepipophyllotoxin-4-(4,6-O-thenylidene-beta-D- glucopyranoside) (VM-26)] is a cancer chemotherapeutic drug with a high target specificity for DNA topoisomerase II. This agent induces repairable protein-bridged double-strand DNA breaks, which have been correlated with cytotoxicity, but high concentrations of VM-26 also induce irreversible DNA degradation and apoptotic cell ...