Five new species belonging to the poorly known Neotropical doryctine parasitoid wasps genera Heerz Marsh (Heerz ecmahlasp. n. and Heerz macrophthalmasp. n.), Lissopsius Marsh (Lissopsius pacificussp. n. and Lissopsius jalisciensissp. n.) and Ondigus Braet, Barbalho & van Achterberg (Ondigus cuixmalensissp. n.) are described from the Chamela-Cuixmala Biosphere reserve in Jalisco, Mexico. Keys to...

Fragments of amplified Xenopus laevis DNA, coding for 18S and 28S ribosomal RNA and generated by EcoRI restriction endonuclease, have been linked in vitro to the bacterial plasmid pSC101; and the recombinant molecular species have been introduced into E. coli by transformation. These recombinant plasmids, containing both eukaryotic and prokaryotic DNA, replicate stably in E. coli. RNA isolated ...

The effect of protein synthesis inhibition by cycloheximide on nucleolar RNA synthesis and processing has been studied in HeLa cells. Synthesis of 45S RNA precursor falls rapidly after administration of the drug. However, the nucleolar content of 45S RNA remains relatively constant for at least 1 hr because the time required for cleavage of the precursor molecule into its products is lengthened...

The kinetics of appearance of newly made 60S and 40S ribosomal subunits in the free and membrane-bound ribosomal particles of P3K cells were explored by determining the specific radioactivities of their 18S and 28S RNA after various lengths of [3H]uridine pulse. Both 40S and 60S subunits enter free and membrane-bound polyribosomes at comparable rates from the cytoplasmic pool of newly made, fre...

Species of the monogenean genus Thaparocleidus are specific to freshwater siluriform fish. The infection caused by these gill parasites are a major health problem to fish. But, to focus the control strategies of these parasites, first it is important to establish an accurate discrimination by molecular methods. In the present study, phylogenetic and structural analysis of 28S region of ribosoma...

We have analyzed multiple recombinant DNA clones containing ribosomal RNA repeat units of the silkmoth, Bombyx mori. In combination with genomic DNA blots, analysis of these clones indicated that the rDNA repeat of B. mori is 10.8 kilobase pair in length and tandemly repeated in the genome, as reported by Manning et al. (18). However, contrary to that report, approximately 12% of the rDNA cistr...

Fungi have unique properties and are used in many areas of agriculture industry because they can produce different enzymes. This study aims to the fungal diversity peat soil from Pontian Johor, Malaysia. The isolates were described on culture media a new medium called EVA identified using phenotypical characteristics molecular D1/D2 domain 28S large subunit ribosomal RNA (28S rRNA) ITS (ITS1-IT...