background acinetobacter calcoaceticus baumannii (acb) complex are gram-negative opportunistic bacteria with low virulence properties. their resistance to antibiotics has become a matter of concern in hospital infections. objectives the present study aimed to determine the prevalence and antimicrobial susceptibility of acb isolates collected from the nemazee hospital of shiraz. in addition, pul...

In more than 20 years of the proteomics era, considerable technical developments and scientific discoveries have contributed to the advancement of this field of research. Gel-based proteomics has been popular for studying the proteomic changes during growth and development of plants as well as for the analysis of responses to different biotic and abiotic stimuli. The most widely used gel-based ...

One of the most important tasks performed in proteomics is correct analysis and interpretation of 2D electrophoresis gel images. The nonlinearities in the gel formation and image acquisition process leads to distortions, overlapped protein spots, saturated spots, faint spots, nonlinear intensity and uneven background in the gel images. As a result, pre-processing and post processing steps are v...

The mitochondrial genome exists in numerous structural conformations, complicating the study of mitochondrial DNA (mtDNA) metabolism. Here, we describe the development of 2D intact mtDNA agarose gel electrophoresis (2D-IMAGE) for the separation and detection of approximately two-dozen distinct topoisomers. Although the major topoisomers were well conserved across many cell and tissue types, uni...