Glycerol dehydrogenase (GDH) is an important polyol dehydrogenase for glycerol metabolism in diverse microorganisms and for value-added utilization of glycerol in the industry. Two GDHs from Klebsiella pneumoniae, DhaD and GldA, were expressed in Escherichia coli, purified and characterized for substrate specificity and kinetic parameters. Both DhaD and GldA could catalyze the interconversion o...

BACKGROUND Paenibacillus polymyxa is a facultative anaerobe known for production of hydrolytic enzymes and various important biofuel molecules. Despite its wide industrial use and the availability of its genome sequence, very little is known about metabolic pathways operative in the Paenibacillus system. Here, we report metabolic insights of an insect gut symbiont, Paenibacillus polymyxa ICGEB2...

Manipulation of NADH-dependent steps, and particularly disruption of the las-located lactate dehydrogenase (ldh) gene in Lactococcus lactis, is common to engineering strategies envisaging the accumulation of reduced end products other than lactate. Reverse transcription-PCR experiments revealed that three out of the four genes assigned to lactate dehydrogenase in the genome of L. lactis, i.e., ...

Twenty Karakul rams were allotted to four groups, subjected first to starvation and then allowed restricted amounts (approximately 1/3 of ad l ib i tum intake) of a high fiber, low energy diet containing no additive (control), 5% 1,3-butanediol (BD), 10% BD, and 5% 1,2-propanediol (PD) for 6 weeks. Starvation elevated (P<.O01) serum ketones (3-hydroxybutyrate and acetoacetate) and decreased (P<...

Bacterial cellulose (BC) is a good material candidate for wound dressing because of its fine 3-D network structure, high mechanical strength and water holding capability, biocompatibility. In this study, composite hydrogel was prepared by using 1,4-butanediol diglycidyl ether (BDDE) to cross-link BC hyaluronic acid (HA). Cross-linked BC/HA composites exhibited denser smoother surface. This dens...

In the present study, biocompatibility of native, acellular, 1,4-butanediol diglycidylether and 1-ethyl-3-(3-dimethyl aminopropyl carbodiimide (EDC) cross-linked acellular aortic grafts was evaluated following subcutaneous implantation in guinea pigs. Biocompatibility was evaluated based on macroscopic, histopathological observations and immune responses elicited by the implanted grafts. Result...